实验性水上漂浮和高强度运动对大鼠下丘脑-垂体-睾丸轴功能的影响  

Experimental Navigation and Deuteroexhaustive Exercise Suppress the Function of the Hypothalamic-pituitary-testicle Axis in Rats

作　　者：李洁[1] 王坚[1] 赵旦[1] 胡兰萍[1] 邵加庆[1] 顾萍[1] 王扬天[1] 

机构地区：[1]南京军区南京总医院内分泌科,江苏南京210002

出　　处：《中华男科学杂志》2008年第1期58-61,共4页National Journal of Andrology

基　　金：南京军区"十一五"面上课题(06MA132)

摘　　要：目的:探讨大鼠在经历水上漂浮和高强度运动后血清T、皮质酮(CORT)、LH、FSH的变化规律。方法:将大鼠随机分为对照组(C组)、水上漂浮组(A组)、水上漂浮并高强度运动组(B组)3组,每组12只,实验分为3个阶段:第一阶段为适应性训练阶段,3d,每天10min,对模拟水上漂浮装置和大鼠跑台进行适应性训练;第二阶段为水上漂浮阶段,共3h;第三阶段为水上漂浮并高强度运动阶段,共2h。A组大鼠和B组大鼠分别经历第一、二阶段和第一、二、三阶段。实验结束后测定血清T、CORT、LH、FSH水平。结果:血清T(呈非正态分布,经对数转换后分析)A组为0.17±0.07,B组-1.11±0.35,与C组0.67±0.26相比均显著下降(P<0.05);LHB组为(3.97±1.57)mIU/ml,与C组(6.49±1.56)mIU/ml相比显著下降(P<0.05);FSH无显著变化;CORTB组为(977.22±207.36)ng/ml,与C组(434.58±110.45)ng/ml相比明显上升(P<0.05)。结论:在水上漂浮阶段,大鼠血清T出现明显下降,说明严重的心理应激会抑制下丘脑-垂体-睾丸轴的功能。随着应激原的增多和应激强度的增加,LH明显下降,T进行性下降,说明下丘脑-垂体-睾丸轴功能受到进一步抑制。Objective： To study the effects of experimental navigation and deuteroexhaustive exercise on the serum levels of testoster-one （T）, corticosterone （CORT）, luteinizing hormone （LH） and follicle stimulating hormone （FSH）. Methods： Thirty-six male SD rats were randomly divided into an experimental navigation group （Group A）, which underwent 180 min wearing floating with psychological stress, a deuteroexhaustive exercise group （Group B）, which were subjected to 120 min intensive running on the treadmill after the accomplishment of the same procedure as Group A, and a control group （Group C）. Blood samples were obtained at the end of the experiment to determine the T, CORT, LH and FSH of the rats. Results： Compared with Group C, serum T was statistically decreased in Group A and B （P 〈 0.05）, while CORT was increased slightly in Group A and significantly in Group B （P 〈 0.05 ）.A statistically lower level of serum LH was observed in Group B （P 〈 0.05）, but no significant differences were found in serum FSH among the three groups. Conclusion ： Stresses of experimental navigation and intensive exercise suppress the function of the hypotha-lamic-pituitary-testicle axis in rats.

关 键 词：水上漂浮 跑台 大鼠 睾酮 皮质酮 黄体生成素 卵泡刺激素 

分 类 号：R-332[医药卫生] R58