食管鳞状细胞癌组织中核干细胞因子蛋白和mRNA的半定量表达  

Expression of nucleostemin mRNA and protein in the esophageal squamous cell carcinoma

作　　者：张功员[1] 尹磊[1] 李晟磊[2] 邢文英[1] 赵秋民[2] 乐晓平[1] 高冬玲[2] 陈奎生[2] 张云汉[2] 张钦宪[1] 

机构地区：[1]郑州大学基础医学院组织与胚胎学教研室,450052 [2]郑州大学基础医学院病理学教研室,450052

出　　处：《中华肿瘤杂志》2008年第2期125-128,共4页Chinese Journal of Oncology

基　　金：河南省教育厅自然科学研究资助项目（2007310017）;郑州市科技攻关资助项目（064SGDS33218-28）

摘　　要：目的探讨食管鳞状细胞癌组织中核干细胞因子（NS）蛋白和mRNA的半定量表达情况。方法应用免疫组化SABC法和逆转录聚合酶链反应（RT-PCR）法，检测62例食管鳞状细胞癌组织及其相对应的31例癌旁不典型增生组织和62例正常食管黏膜组织中NS蛋白和mRNA的半定量表达情况。结果正常食管黏膜组织、癌旁不典型增生组织和食管鳞状细胞癌组织中，NS蛋白阳性表达率分别为17．7％（11／62）、41．9％（13／31）和69．4％（43／62），组间比较，差异有统计学意义（X2=33．676，P〈0．01）。食管鳞状细胞癌组织中，Ns蛋白阳性表达率与其组织学分级、侵袭程度及淋巴结转移密切相关（均为P〈0．05），而与年龄、性别和病理分型无关（均为P〉0．05）。食管鳞状细胞癌组织中，NS mRNA的相对含量（0．971±0．121）高于癌旁不典型增生组织（0．913±0．085）和正常食管黏膜组织（0．866±0．103），组间比较，差异有统计学意义（F=14．829，P〈0．01）。不同组织学分级、不同侵袭程度及有无淋巴结转移的食管鳞状细胞癌组织之间，NS mRNA的相对含量差异均有统计学意义（均为P〈0．05），NS mRNA的相对含量与年龄、性别和病理分型无关（均为P〉0．05）。结论食管鳞状细胞癌组织中，NS mRNA的表达升高，其高表达与食管鳞状细胞癌的发生发展有关。Objective To investigate the mRNA and protein expression of nucleostemin （NS） in human esophageal squamous cell carcinoma. Methods The mRNA and protein expression of NS were detected in 31 mucosal atypical hyperplasia specimens, 62 esophageal squamous cell carcinoma specimens and the matched normal esophageal mucosa samples by RT-PCR and immunohistochemistry method, respectively. Results The positive expression rate of NS protein in normal esophageal mucosa, atypical hyperplasia and esophageal squamous cell carcinoma was 17.7% （11/62） , 41.9% （ 13/31 ） and 69.4% （43/62） , respectively. There was a significant difference among the above three groups （x^2 = 33. 676, P 〈 0.01 ）. The expression levels of NS mRNA in esophageal squamous cell carcinoma （0. 971 ± 0. 121 ） was significantly higher than that in the atypical hyperplasia （0. 913 ± 0. 085 ） and also in the normal esophageal mucosa（0. 866 ± 0. 103 ; F = 14. 829, P 〈 0.01 ）. The expression level of both NS protein and mRNA was positively correlated with histological grade, infiltration depth, and lymph node metastasis （P 〈 0.05 ）, but not with age, gender or pathological type （P 〉0.05 ）. Conclusion Our results indicate that nucleostemin mRNA and protein are over-expressed in human esophageal squamous cell carcinoma, and it may be related with its oncogenesis.

关 键 词：食管肿瘤 鳞状细胞癌 核干细胞因子 MRNA 

分 类 号：R735.1[医药卫生—肿瘤]