腈水合酶产生菌的分离筛选和酶反应条件的优化  

Screening of Nitrile Hydratase Producing Bacterium and Optimization of Enzymatic Reaction Condition

作　　者：秦智[1] 蒋忠平[1] 朱婷婷[1] 张云天[1] 孙晓君[1] 

机构地区：[1]哈尔滨理工大学,哈尔滨150040

出　　处：《东北林业大学学报》2008年第2期61-62,72,共3页Journal of Northeast Forestry University

基　　金：哈尔滨市科技攻关项目(2004AA4CS049);哈尔滨市青年科学研究基金资助项目(2004AFQXJ041)

摘　　要：从化工废水处理厂的活性污泥中分离和筛选到一株腈水合酶产生菌HUST-1。经初步鉴定,菌株HUST-1属于红球菌属(Rhodococcus sp.)。对菌株(Rhodococcus sp.)HUST-1所产腈水合酶的反应条件进行研究,结果表明,该菌株的最优酶反应条件:丙烯腈加入量为5%,反应温度为28℃,pH值为7.0,反应时间为15min。在最优酶反应条件下,菌株(Rhodococcus sp.)HUST-1的最高比酶活可达119U·mg-1,比优化前提高24.5%。An experiment was conducted to isolate and screen a nitrile hydratase producing strain HUST-1 from activated sludge in a chemical wastewater treatment plant. Strain HUST-1 was primarily identified as Rhodococcus sp. according to its morphological observation. The optimal nitrile hydratase reaction conditions for Rhodococcus sp. HUST-1 were obtained as acrylonitrile 5%, reaction temperature 28 degrees C, pH 7.0 and reaction time 15 minutes. Under the optima/ conditions, the specific enzyme activity of Rhodococcus sp. HUST-1 reached 119 U · mg^-1, showing an increase by 24.5% than before optimization.

关 键 词：腈水合酶 红球菌属 酶反应条件 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]