大孔吸附树脂分离地菍总酚的研究  

Separation of phenols from Melastoma dodecandrum with macroporous resins

作　　者：翟小玲[1] 倪健[1] 谷雨龙[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《中草药》2008年第2期196-199,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的筛选大孔树脂分离纯化地菍中总酚类物质的最佳工艺。方法采用静态与动态吸附-解吸方法,以紫外分光光度法测定地菍总酚的量对工艺进行评价。结果HPD100大孔吸附树脂分离效果较好,总酚可达50%以上。其最佳工艺为药液浓度0.2g(生药)/mL、pH值为2.7～4.5,以3BV/h进行吸附,5BV的60%乙醇以3BV/h进行洗脱效果最佳。结论该方法简便可行,分离效果好,能满足大生产的要求。Objective To explore the optimal techniques in separation of phenols from Melastoma dodecandrum with macroporous resins. Methods Static and dynamic adsorption-desorption methods were adopted, and the concentration of phenols was measured by UV spectrophotometer to evaluate the separating efficiency. Results The HPD 100 macroporous resin was found with the best separating efficiency, by which the content of phenols could reach more than 50 percent in the extraction. The optimum technique condition was 0.2 g/mL concentration of crude drug, pH value was 2.7-4.5, adsorption in 3 BV/ h, delution by 5 BV, 60% alcohol in 3 BV/h. Conclusion This method is simple and feasible with good efficiency of separation, which could also meet the industrial requirements.

关 键 词：地苍 总酚 大孔吸附树脂 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]