胶原蛋白多肽铬对四氧嘧啶损伤肝细胞的保护作用  

Protective Effect of Collagen Polypeptide-chromium Chelate in Alloxan-treated HL 7702 Hepatocytes

作　　者：刘安军[1] 陈影[1] 张国蓉[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《现代食品科技》2008年第2期101-104,共4页Modern Food Science and Technology

基　　金：天津市自然科学基金(No.013617111)

摘　　要：研究了胶原蛋白多肽铬(CPCC)对四氧嘧啶损伤肝细胞HL-7702的保护作用,并探讨其作用机理。方法是将肝细胞HL-7702分为正常对照组、四氧嘧啶组、胶原蛋白多肽铬组及四氧嘧啶+胶原蛋白多肽铬组,分别检测细胞存活率、细胞胞内活性氧(ROS)、胞外超氧阴离子(O2.-)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力、脂质过氧化物丙二醛(MDA)含量,并检测胶原蛋白多肽铬在过氧化氢(H2O2)模型组中的作用。结果表明胶原蛋白多肽铬处理使四氧嘧啶损伤肝细胞存活率增加,胞内ROS生成减少,O2.-含量、SOD与GSH-Px低偿性升高及MDA含量降低。结论是胶原蛋白多肽铬处理对四氧嘧啶损伤肝细胞具有一定的保护作用,其作用机制可能与三价铬抗氧化性能有关。To study the protective effect of collagen polypeptide-chromium chelate （CPCC） in HL 7702 hepatocytes treated by alloxan and the possible mechanism. Cells were divided into control group, alloxan-treated group, CPCC group and alloxan＋CPCC group. Cell viability, contents of reactive oxygen species （ROS） and superoxide radical （O2）, activities of SOD and GSH-Px and MDA content were analyzed. The protective effect of CPCC in H2O2-treated hepatices was also determined. Results shows that Cell viability was significantly increased by CPCC, while the contents of ROS, O2 and MDA and the activities of SOD and GSH-Px in alloxan-treated hepatocyte were decreased. CPCC had a protective effect in alloxan-treated HL 7702 hepatocytes, acting as an antioxidant.

关 键 词：胶原蛋白多肽铬 HL-7702人肝细胞 四氧嘧啶 抗氧化 

分 类 号：R96[医药卫生—药理学]