Toxicity assessment of heavy metals and organic compounds using CellSense biosensor with E.coli  

作　　者：Hong Wang Xue Jiang Wang Jian Fu Zhao Ling Chen 

机构地区：[1]State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, ongji University, Shanghai 200092, China

出　　处：《Chinese Chemical Letters》2008年第2期211-214,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.20707014);the Program for Young Excellent Talents of Tongji University.

摘　　要：A new strategy using an arnperometric biosensor with Escherichia coli （E. coli） that provides a rapid toxicity determination of chemical compounds is described. The CellSense biosensor system comprises a biological component immobilized in intimate contact with a transducer which converts the biochemical signal into a quantifiable electrical signal. Toxicity assessment of heavy metals using E.coli biosensors could be finished within 30 min and the 50% effective concentrations （ECso） values of four heavy metals were determined. The results shows that inhibitory effects of four heavy metals to E.coli can be ranked in a decreasing order of Hg^2＋ 〉 Cu^2＋ 〉 Zn^2＋ 〉 Ni^2＋, which accords to the results of conventional bacterial counting method. The toxicity test of organic compounds by using CellSense biosensor was also demonstrated. The CellSense biosensor with E. coli shows a good, reproducible behavior and can be used for reproducible measurements.A new strategy using an arnperometric biosensor with Escherichia coli （E. coli） that provides a rapid toxicity determination of chemical compounds is described. The CellSense biosensor system comprises a biological component immobilized in intimate contact with a transducer which converts the biochemical signal into a quantifiable electrical signal. Toxicity assessment of heavy metals using E.coli biosensors could be finished within 30 min and the 50% effective concentrations （ECso） values of four heavy metals were determined. The results shows that inhibitory effects of four heavy metals to E.coli can be ranked in a decreasing order of Hg^2＋ 〉 Cu^2＋ 〉 Zn^2＋ 〉 Ni^2＋, which accords to the results of conventional bacterial counting method. The toxicity test of organic compounds by using CellSense biosensor was also demonstrated. The CellSense biosensor with E. coli shows a good, reproducible behavior and can be used for reproducible measurements.

关 键 词：Whole-cell biosensor Heavy metal Organic compounds TOXICITY 

分 类 号：TQ2[化学工程—有机化工]