固定化嗜热菌蛋白酶合成前体α-ZAPM的研究  

Synthesis of Aspartame Precursor-α-ZAPM Catalyzed by Immobilized Thermolysin

作　　者：吴梧桐[1] 严悦日[1] 

机构地区：[1]中国药科大学生化教研室,南京210009

出　　处：《药物生物技术》1997年第2期65-71,共7页Pharmaceutical Biotechnology

摘　　要：以固定化嗜热菌蛋白酶催化合成天苯二肽前体α- ZAPM,再脱去保护基后得到天苯二肽。固定化优化条件为 :海藻酸钠起始浓度 4 % ,戊二醛 0 .5% ,交联时间 10 min,凝胶球直径 2 .0至 2 .5mm。固定化酶最适反应 p H为 7.0 ,最适反应温度为 30℃。固定化酶对 p H和温度的稳定性明显提高 。Immobilized thermolysin catalyzed synthesis of aspartame precursor α ZAPM, and aspartame was obtained after removal of protecting group. Optimal immobilization conditions included: initial concentration of sodium alginate 4%; concentration of glutaraldenyde 0.5%; crosslinking time 10 minutes; diameter of gel ball 2.0～2.5mm. Optimal reaction pH of immobilized enzyme was 7.0, optimal reaction tmperature was 30℃. Stability of immobilized enzyme to variations of pH and temperature was higher than that of free enzyme. Immobilized enzyme can be reutilized to synthesize α ZAPM through batch reactions.

关 键 词：嗜热菌 蛋白酶 固定化酶 α-ZAPM 天苯二肽 

分 类 号：Q556.3[生物学—生物化学]