腺病毒介导的野生型p53基因对肺腺癌细胞株生长的抑制作用  

The Role of Recombinant p53 Adenovirus Vector in the Growth of Lung Adenocarcinoma Cell Line GLC-82

作　　者：夏永静[1] 蒋雷[1] 李红霞[1] 黎健[1] 胡亚军[2] 衣林[2] 

机构地区：[1]卫生部北京老年病研究所,北京100730 [2]北京医院肿瘤内科

出　　处：《中国肿瘤生物治疗杂志》1997年第2期104-106,共3页Chinese Journal of Cancer Biotherapy

基　　金：本文受国家自然科学基金(39570775)资助

摘　　要：为研究野生型p53基因对肺腺癌细胞株GLC-82细胞生长的作用,确立腺病毒介导的野生型p53基因在肿瘤治疗中的意义,应用分子克隆技术首先构建了野生型p53复制缺陷型腺病毒重组体,应用生化染色方法确定了重组体的转染效率,以免疫组化法及PCR技术分别确定了腺病毒载体介导的p53基因转染GLC-82细胞后p53蛋白和p53 cDNA的表达情况;最后应用细胞生物学方法检测了p53对GLC-82细胞扩增及细胞周期的影响.结果表明所构建的重组体可以高效、快速转染GLC82细胞,抑制GLC-82细胞扩增,使细胞合成期和分裂后期的量减少。Wild-type p53 has been shown to be involved in several aspects of cell growth control. In order to study the role of wild-type p53 in the growth of lung adenocarcinoma cell line GLC-82, recombinant p53 adenovirus vector and the control LacZ gene were constructed. Infection conditions were detected by biochemistry staining for LacZ s expression product β-gal, immunohistochemical analysis for p53 protein expression, and PCR technique for the infected cells. The cell growth rate and cell cycle were analysed with flow cytometry. The results showed that the constructed recombinant adenovirus vector could infect cells with high level expression of (β-gal and p53 protein and p53 cDNA could be found in the infected cells. The wild-type p53 could inhibit GLC-82 cell proliferation and cause cellular death. These results indicated that wild-type p53 gene mediated by adenovirus vector might be used in the treatment of lung adenocarcinoma.

关 键 词：腺病毒载体 P53基因 肺肿瘤 腺癌细胞株 

分 类 号：R734.2[医药卫生—肿瘤]