Apelin-13促大鼠血管平滑肌细胞增殖的PKC-ERK1/2信号通路研究  

The research of PKC-ERK1/2 signaling transduction pathway of rat VSMCs proliferation induced by apelin-13

作　　者：潘伟男[1] 封芬[1] 陈锋[1] 秦旭平[1] 朱炳阳[1] 李峰[1] 李兰芳[1] 陈临溪[1] 

机构地区：[1]南华大学药物药理研究所,湖南衡阳421001

出　　处：《中国药理学通报》2008年第2期214-218,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30371645);湖南省教育厅优秀青年基金资助项目(No03B036)

摘　　要：目的研究G蛋白偶联受体APJ(血管紧张素受体样受体或称血管紧张素受体AT1相关的受体蛋白,putativere-ceptor protein related to the angiotensin receptor AT1)的内源性配体apelin-13通过PKC-ERK1/2-Cyclins信号通路促进大鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖的影响。方法培养SD大鼠胸主动脉VSMCs,Western blot检测p-ERK1/2、ERK1/2、细胞周期蛋白CyclinD1和CyclinE的表达,四噻唑蓝比色法观察PKC阻断剂GF109203X对apelin-13促大鼠VSMCs增殖的影响。结果Apelin-13剂量依赖性和时间依赖性地促进大鼠VSMCsp-ERK1/2表达增加,对ERK1/2表达没有明显影响,GF109203X可明显抑制apelin-13诱导的细胞增殖及p-ERK1/2、CyclinD1和CyclinE的表达。结论Apelin-13促进大鼠VSMCs增殖可能与ape-lin-APJ-PKC-ERK1/2-Cyclins信号通路有关。Aim To investigate the effects of cell proliferation induced by apelin-13 via PKC-ERK1/2-Cyclins signaling pathway in rat vascular smooth muscle cells（VSMCs）. Further to find new signaling pathway of apelin-APJ. Methods vSMCs were prepared from male Sprague-Dawley rats＇thoracic aortas by the primary-explant method. Expression of p-ERK1/2, ERK1/2, CyclinD1 and CyclinE was detected by Western blot. The blockade effects of GF109203X were measured by MTT assay. Results Apelin-13 promoted the concentration-dependent and time-dependent expression of p-ERK1/2 during 2μmol ·L^-1 and 4 h. The potent PKC inhibitor GF109203X decreased the expression of p-ERK1/2 ,CyclinD1 and CyclinE. MTY assay showed that GF109203X significantly inhibited the VSMCs proliferation stimulated by apelin-13. Conclusions The effects of apelin-13 promoted cell proliferation maybe involved in apelin-APJ-PKC-ERK1/2-Cyclins signal cascades in rat VSMCs.

关 键 词：APELIN APJ 血管平滑肌细胞 细胞周期蛋白 P-ERK1/2 

分 类 号：R-332[医药卫生] R322.74