人参皂苷预防大鼠心肌肥大的评价  

Evaluation on ginsenoside preventing pressure-overload hypertrophy in rats

作　　者：张凌志[1] 邸菁[2] 柏树令[2] 张海平[3] 刘昉[2] 

机构地区：[1]中国医科大学附属盛京医院急诊科,辽宁沈阳110004 [2]中国医科大学人体解剖学教研室,辽宁沈阳110001 [3]温州医学院附属第二医院超声科,浙江温州325027

出　　处：《中国药理学通报》2008年第2期233-236,共4页Chinese Pharmacological Bulletin

基　　金：辽宁省科技计划项目资助课题(No2005225007-1)

摘　　要：目的评价人参皂苷对心肌肥大的预防作用。方法采用升主动脉缩窄的方法制作大鼠压力负荷心肌肥大动物模型,同时用人参皂苷进行预防性治疗,通过三维彩色超声技术和免疫荧光双重染色的方法评价人参皂苷的治疗作用。结果超声学指标,与假手术组相比,心肌肥大组的LVPW、IVS增加(P<0.01),LVDD减小(P<0.01);而与心肌肥大组相比,人参皂苷组LVPW、IVS减少(P<0.01),LVDD增加(P<0.01)。形态学上,人参皂苷能逆转心肌纤维肥大和减少间质增生。结论人参皂苷在早期能预防或延缓心肌肥大的发生。Aim To observe the effects of Ginsenoside on left ventricular remodeling after pressure-overload hypertrophy in rats. Methods After stenosis of the ascending aortic artery, 20 survived female Wistar rats were randomly assigned to two groups： hypertrophy control （n = 10） and Ginsenoside（ 100 mg · kg· d^-1,n = 10） ; sham operated rats （ n = 10 ） were selected randomly as nonstenosis control. Four weeks after the operation, the LVPW, IVS and LVDD of each rat were detected by echocardiogram. Myocardial cell and inter- stitial tissue were observed by immunofluorescence double staining. Results Compared with those in hypertrophy group, the LVPW and IVS in Ginsenoside group were all significantly decreased（ P 〈0. 01 ）. The LVDD was significantly increased （ P 〈 0. 01 ）. Meanwhile, cardiac myocyte hypertrophy was found by immunohistochemistry staining. Conclusion Ginsenoside can prevent or delay the development of hypertrophy.

关 键 词：人参皂苷 心肌肥大 压力负荷 三维彩超 免疫荧光染色 

分 类 号：R-332[医药卫生] R284.1