银杏内酯B对缺血／再灌脑损伤大鼠的保护作用  

Protective effects of ginkgolide B on cerebral ischemia reperfusion injury in rats

作　　者：黄贱英[1] 孙建宁[2] 梅世昌[3] 黄纪明[1] 

机构地区：[1]中国航天员科研训练中心,北京100094 [2]北京中医药大学,北京100102 [3]中国人民解放军总医院,北京100102

出　　处：《中国药理学通报》2008年第2期269-272,共4页Chinese Pharmacological Bulletin

摘　　要：目的探讨银杏内酯B对缺血/再灌注脑损伤大鼠的保护作用及其可能的作用机制。方法通过颈总动脉栓线造成大脑中动脉缺血,缺血3h后将线拔出以实现大脑中动脉血流再灌注,同时观察银杏内酯B对大鼠神经症状,脑梗死范围以及银杏内酯B对缺血侧脑组织匀浆SOD活力,MDA含量,LDH活力及LD含量的影响。并且进行了病理组织切片检查。结果银杏内酯B对缺血3h大鼠神经症状无影响,对再灌21h大鼠神经症状有明显的改善作用。银杏内酯B10、5mg·kg-1能明显降低脑梗死范围,同时银杏内酯B能升高SOD活力,减少MDA含量及LDH活力,但对LD含量无明显作用。病理切片检查结果表明,银杏内酯B能减轻缺血侧大脑的水肿及空泡现象。结论银杏内酯B对缺血/再灌注脑损伤有保护作用,该作用可能与其升高SOD活力,降低MDA含量及LDH活力有关。Aim To observe the protective effects of ginkgolide B（GB） on cerebral ischemia-reperfusion injury in rats and explore its mechanism. Methods Cerebral ischemia-reperfusion injury in rats was induced by middle cerebral artery occlusion. The neurological out-come and infarct size were evaluated. SOD and LDH activity, LD and MDA levels of ischemia cerebral tissue were measured. The pathomorphology examination was done. Results GB did not improve neurologic defects in 3 h ischemia rats, but it improved neurologic defects in 21 h reperfusion rats. GB （10, 5 mg·kg^-1 ） markedly decreased infarct size in rats. SOD activity in GB（10,5 mg ·kg^-1 groups was higher, and LDH activity and MDA level in GB （10,5,2.5 mg·kg^-1 ） groups were significantly lower than those in vehicle group. LD level did not chang. The cellular and intercellular edema was reduced markedly in the pathomorphology examination. Conclusions GB has protective effects on cerebral ischemia-reperfusion injury in rats. Its role is probably related to its effect on the activity of SOD and LDH, and the level of MDA.

关 键 词：银杏内酯B 脑缺血 再灌注损伤 

分 类 号：R-332[医药卫生] R284.1