神经生长因子缓释微球移植对AD模型鼠基底前脑ChAT阳性神经元的保护作用  

Effect of implantation of rhngf microspheres on the chat-positive neurons of the basal forebrain after fornix-fimbria transections

作　　者：谷海刚[1] 龙大宏[2] 宋存先[3] 李晓滨[1] 

机构地区：[1]广州医学院组织胚胎学教研室,广东广州510182 [2]广州医学院人解剖学教研室,广东广州510182 [3]中国医学科学院北京协和医学院生物医学工程研究所,天津300192

出　　处：《解剖学研究》2008年第1期47-51,共5页Anatomy Research

摘　　要：目的观察神经生长因子微球对AD模型鼠基底前脑ChAT阳性神经元的保护作用。方法采用双乳化技术制备神经生长因子缓释微球;切断SD大鼠左侧穹隆海马伞,基底前脑注射神经生长因子缓释微球;4周后,利用免疫组化法观察各组大鼠基底前脑ChAT阳性神经元变化。结果损伤组损伤侧的MS和VDB的ChAT阳性神经元大量减少,分别减少61.9%和51.4%;神经生长因子缓释微球治疗组损伤侧的ChAT阳性神经元得到明显的保护,MS和VDB细胞数分别下降20.60%和20.9%,明显高于损伤组损伤侧的ChAT阳性神经元存活数。结论神经生长因子缓释微球能够成功地将神经生长因子运载到脑内,神经生长因子缓释微球移植对AD模型鼠基底前脑ChAT阳性神经元有明显的保护作用。Objective To study the effect of implantation of rhNGF microspheres on the ChAT-positive neurons of the basal forebrain after fornix-fimbria transactions. Methods Recombinant human NGF microspheres were prepared by a W/O/W emulsion and solvent evaporation technique with some modifications. Recombinant human NGF microspheres were transplanted into the basal forebrain after fornix-fimbria transactions. After 4 weeks, ChAT-positive neurons of the basal forebrain in the different groups were observed by immunohistochemistry. Results After 4 weeks, in LES group, the percentages of CHATpositive neurons at the lesion side to the intact side of MS and VDB were 38.1% and 48.6%, respectively. In MIC group, the percentages of ChAT-positive neurons at the lesion side to the intact side of MS and VDB were 79.4% and 79.1%, respectively, which were significantly more than that in LES group （P〈0.01）. Conculsions NGF can be successfully deliveried to the brain by recombinant human NGF microspheres. Recombinant human NGF microspheres promote the survival of ChAT-positive neurons of the basal forebrain after fornix-fimbria transections.

关 键 词：老年性痴呆 神经生长因子微球 ChAT阳性神经元 基底前脑 大鼠 

分 类 号：R749.16[医药卫生—神经病学与精神病学]