苦参碱诱导多发性骨髓瘤RPMI8226细胞凋亡及其对细胞黏附分子表达的影响  

Effects of Matrine on the Apoptosis and Expression of Adhesion Molecule in Multiple Myeloma RMPI8226 Cells

作　　者：吴建波[1] 章圣辉[1] 韩义香[1] 熊术道[1] 叶爱芳[1] 谭映霞[1] 

机构地区：[1]温州医学院附属第一医院医学科学研究所,浙江温州325000

出　　处：《中国实验血液学杂志》2008年第1期93-96,共4页Journal of Experimental Hematology

摘　　要：为了探讨苦参碱诱导多发性骨髓瘤(MM)RPMI8226细胞凋亡及其对细胞黏附分子表达的影响,将苦参碱与RPMI8226细胞共培育,采用CCK-8法观察细胞生长,Annexin V-FITC/PI双染色流式细胞仪检测细胞凋亡,PI单染色法检测细胞周期,双染色流式细胞仪检测细胞膜表面CD44、CD44v6、CD54和CD106的表达。结果表明,苦参碱对RPMI8226细胞具有明显的生长抑制作用,呈量效和时效关系;苦参碱能诱导RPMI8226细胞凋亡,其发生率随着药物浓度增加而增加;细胞周期分析显示G0/G1期细胞相对增多,S期相对减少,但G2/M期细胞数无明显改变;苦参碱作用于RPMI8226细胞48小时导致CD44和CD54表达减少,但CD44v6和CD106表达无明显改变。结论:苦参碱通过诱导凋亡和细胞周期阻滞抑制RPMI8226细胞的生长,同时降低CD44、CD54等细胞黏附分子表达。To investigate the effects of matdne on apoptosis and expression of adhesion molecules in human mutiple myeloma cell line RPMI8226 cells, RPMI8226 cells were incubated with indicated concentrations of matrine. The growth of RPMI8226 cells was observed by CCK-8 colorimetric assay and apoptosis was detected by flow cytometry using Annexin V-FTTC/PI staining. The cell cycles were analyzed by PI staining. Flow cytometry using Annexin V-FTTC/PI staining was used to detect the expression of cell adhesion molecules, including CD44, CD44v6 ,CD54 and CD106. The results showed that RPMI8226 cell viability in presence of matrine decreased markedly in a dose- and time-dependent manners. The apoptosis could be induced by matrine and its level increased following the augmentation of the drug concentration. After treated by matrine for 48 hours, a concentration-dependent increase of cells in G0/G1 phase and a decrease in S phase could be detected, but no obvious change of cell count was found in G2/M phase. Treatment of RPMI8226 cells with matrine for 48 hours resulted in decrease of expression levels of CD44 and CD54, while expressions of CD44v6 and CD106 had no significant change. It is concluded that matrine induces in vitro apoptosis, suppresses proliferation in multiple myeloma cells and depresses expression of some adhesion molecules.

关 键 词：苦参碱 骨髓瘤 RPMI8226细胞 细胞凋亡 细胞周期 细胞黏附分子 

分 类 号：R733.3[医药卫生—肿瘤]