应用蛋白质组学方法研究低剂量辐射对人骨髓间充质干细胞的影响  

Effect of Low Dose Radiation on Humam Bone Marrow Mesenchymal Stem Cells by Using Proteomic Analysis

作　　者：张一琼[1] 李薇[1] 王冠军[1] 杨岩[1] 王杰[2] 张学敏[2] 

机构地区：[1]吉林大学第一医院血液肿瘤科,吉林长春130021 [2]国家生物医学分析中心,北京100850

出　　处：《中国实验血液学杂志》2008年第1期151-155,共5页Journal of Experimental Hematology

基　　金：国家自然基金资助项目(编号30370439);教育部高等学校博士学科点专项科研基金资助(编号20020183015)

摘　　要：本研究应用蛋白质组学方法探讨低剂量辐射对人骨髓间充质干细胞的影响。利用双向凝胶电泳建立低剂量照射组与假照组人骨髓间充质干细胞组蛋白质组二维凝胶电泳图谱,利用MALDI-TOF-MS生物质谱对差异表达的蛋白质进行鉴定分析。结果发现,低剂量照射后人骨髓间充质干细胞中表达上调的蛋白有12个,表达下调的蛋白有12个,消失的蛋白有3个。质谱鉴定出12个差异蛋白。结论:鉴定出的12种蛋白,其中某些蛋白如脯氨酰羟化酶、二氢嘧啶酶、ARP3放射相关蛋白、鸟嘌呤核苷酸结合蛋白、磷酸甘油酸变位酶等可能与低剂量辐射作用机制相关,本研究为低剂量辐射对造血系统的作用机制提出了一些新的认识。This study was aimed to investigate the effect of low dose radiation （LDR） on human bone marrow mesenchymal stem cells （MSCs） by using proteomic analysis. The bidirectional gel electrophoresis was used to establish the two-dimensional gel electrophoresis patterns of proteome in group of MSCs exposed to LDR and in group of sham irradiated MSCs, the matrix assisted laser desorption/ionization-time of flight-mass spectrometry （MALDI-TOF-MS） was used to identify the differentially expressed proteins in two groups, The results showed that among the differentially expressed proteins in the two groups, the expressions of 12 proteins were up-regulated, the expressions of 12 protein were down-regulated, 3 proteins disappeared after LDR, 12 proteins had been identified by MALDI-TOF-MS. In conclusion, the identified 12 proteins, such as prolyl 4-hydroxylase, dihydropyrimidinase-like 2 variant, ARP3 （actin- related protein 3, yeast） homolog, guanine nucleotide binding protein （G protein）, phosphoglycerate mutase 1 may be related to mechanism of LDR effect. The study provides some new explanation for the mechanism of low dose radiation injury.

关 键 词：蛋白质组 低剂量辐射 骨髓间充质干细胞 二维凝胶电泳 质谱 

分 类 号：R811.5[医药卫生—放射医学] R311.14[医药卫生—临床医学]