不同融合液对延边黄牛体细胞核移植效率的影响  

Influences of Different Fusion Liquids on the Somatic Cell Nuclear Transfer Efficiency in Yanbian Yellow Cattle

作　　者：李晓霞[1] 刁云飞[1] 李钟淑[1] 方南洙[1] 金长松 

机构地区：[1]延边大学农学院,吉林龙井133400 [2]吉林省龙井市智新镇畜牧兽医站,吉林龙井133400

出　　处：《安徽农业科学》2008年第4期1454-1456,共3页Journal of Anhui Agricultural Sciences

基　　金：振兴东北老工业基地项目;延边州延边大学合作项目

摘　　要：[目的]探寻适宜延边黄牛体细胞核移植的最佳融合液。[方法]用延边黄牛颗粒细胞为供体细胞。试验1,比较3种浓度(0.25、0.280、.30 mol/L)3种融合液(蔗糖、D-山梨醇、甘露醇)对核移植胚胎融合率及重组胚体外发育的影响。试验2,比较3种0.28 mol/L融合液对核移植效率的影响。[结果]3种浓度的蔗糖、D-山梨醇、甘露醇融合液的融合率和卵裂率差异均不显著,但0.28 mol/L融合液处理的重组胚后期发育相对较好。3组融合液融合率(88.44%、86.83%、86.85%)差异不显著,蔗糖、甘露醇融合液卵裂率(87.98%、86.06%)差异不显著,在0.05水平上显著高于D-山梨醇融合液(78.64%);蔗糖、甘露醇融合液囊胚发育率(22.16%、19.91%)差异不显著,在0.05水平上显著高于D-山梨醇融合液(10.14%)。[结论]适宜延边黄牛体细胞核移植的最佳融合液为0.28 mol/L蔗糖融合液。[ Objective] The study aimed to find the best fusion liquid suitable for somatic cell nuclear transfer in Yanbian yellow cattle. [ Method] With granulosa cells from Yanbian yellow cattle as donor cells, the influences of 3 fusion liquids （ D-sorbitol and mannitol） with 3 concentrations （0.25, 0.28 and 0.30 mol/L） on the fusion rate of nuclear transfer embryos and in vitro development of reconstructed embryos were compared in experiment 1. The influences of 3 kinds of 0.28 mol/L fusion liquids on nuclear transfer efficiency were compared in experiment 2. [Result] The differences of fusion rates and cleavage rates among sucrose, D-sorbitol and mannitol fusion liquids with 3 concentrations were not significant. But the anaphase developments of reconstructed embryos in 0.28 ml/L fusion liquid treatment was relatively better. The difference of fusion rates anong 3 groups of fusion liquids （88.44%, 86.83% and 86.85%） was not significant. The cleavage rates of sucrose and mannitol fusion liquids （87.98% and 86.06%） had no significant difference, and were significantly higher than that of D-sorbitol fusion liquid （ 78.64 % ） at 0.05 level, The blastocyst development rates of sucrose and mannitol fusion liquids （22.16% and 19.91 % ） had no significant difference, and were signifieanfly higher than that of D-sorbitol fusion liquid （10.14%） at 0.05 level. [Conclusion] The best fusion liquid suitable for somatic cell nuclear transfer in Yanbian yellow cattle was sucrose fusion hquid at 0.28 mol/L.

关 键 词：延边黄牛 体细胞核移植 融合液 重组胚 体外发育 

分 类 号：S823.81[农业科学—畜牧学]