MTT分析法检测沙立度胺对乳腺癌细胞增殖的作用  

Studies on MTT colorimetric assay on the effect of thalidomide on proliferation of breast cancer cell

作　　者：杨志勇[1] 李书芹[1] 季艳霞[1] 李海燕[1] 张振军[1] 贾军刚[1] 万良刚[1] 

机构地区：[1]河北省邯郸市中心医院放疗科,056001

出　　处：《中国实用医药》2008年第7期11-12,共2页China Practical Medicine

摘　　要：目的观察沙立度胺对乳腺癌细胞MCF-7和MDA-MB-231增殖的抑制作用。方法设置溶剂对照及沙立度胺不同浓度组、时间组,采用四甲基偶氮唑蓝(MTT)法检测各组细胞存活和生长情况,分析量效关系、时效关系。结果①在10～200μg/ml浓度范围,沙立度胺对MCF-7和MDA-MB-231细胞增殖均有抑制作用。在药物作用48h后各组的细胞抑制率均达到高峰,48h时又以100μg/ml浓度组抑制率最高。结论在一定浓度范围,沙立度胺对MCF-7和MDA-MB-231细胞增殖具有抑制作用。Objective To investigate the effect of thalidomide on proliferation in breast cancer cell lines. Methods Establishing the melting agent checks against and different density, establishing different times at the same time, in order to observes the relation between time and efficacy, the growth rate among these groups was detected by MTT assay. Results The growth rate in these groups had differences, thalidomide can inhibit the proliferation of MCF-7 and MDA-MB-231 among 10-200 μg/ml. The beast inhibitory effect is occurring after thalidomide treated 48 h. 100 μg/ml have the beast inhibitory effect after treated 48 h. Conclusion Thalidomide could inhibit the proliferation of breast cancer cells.

关 键 词：乳腺肿瘤 细胞系 沙立度胺 MTT 

分 类 号：R737.9[医药卫生—肿瘤]