荧光定量RT-PCR检测去下颌下腺大鼠睾丸annexin 5 mRNA和Bax mRNA的表达  

THE EXPRESSIONS OF ANNEXIN 5 mRNA AND BAX mRNA WITH FQ-RTPCR IN THE RAT TESTIS WITH SUBMANDIBULAR GLAND REMOVED

作　　者：王晨阳[1] 马百坤[1] 侯林[1] 徐会茹[1] 黄祝[1] 蒋超[1] 黄宇烽[1] 姚兵[1] 

机构地区：[1]南京军区南京总医院解放军临床检验医学研究所

出　　处：《解剖学报》2008年第1期106-110,共5页Acta Anatomica Sinica

基　　金：江苏省自然科学基金(BK2006135);全军“十一五”医药卫生科研基金(06H011)

摘　　要：目的观察下颌下腺切除对大鼠睾丸膜联蛋白5(annexin 5)和Bax mRNA表达的影响。方法切除大鼠下颌下腺,分别于术后14d、28d和42d处死大鼠,提取睾丸总RNA,反转录,设计特异性引物和Taqman探针,荧光定量RT-PCR检测annexin 5和Bax mRNA的表达。结果荧光定量PCR结果显示,与对照组相比,切除下颌下腺14d和28d后,实验组大鼠睾丸annexin 5 mRNA分别升高了38.5%和55.3%,但无显著性差异;实验组大鼠睾丸Bax mRNA分别升高了70.6%和80.5%,其升高具有显著性差异(P<0.05和P<0.01);切除下颌下腺42d时,实验组大鼠睾丸annexin 5和Bax mRNA分别升高5.6%和2.3%,几乎恢复到与对照组相同的水平。结论下颌下腺切除后14d和28d可造成大鼠睾丸annexin 5和Bax mRNA的表达升高;切除后42d,annexin 5和Bax mRNA又恢复到正常水平。Objective To detect the expressions of annexin 5 mRNA and Bax mRNA in the rat testis after the submandibular gland was removed. Methods On days 14, 28 and 42 after the operation, the expression levels of annexin 5 and Bax mRNA in the rat testis were examined by fluorogenic quantitative RT-PCR. Results Fluorogenic quantitative RT-PCR showed that there was a 38.5% and 55.3% increase in the expression of annexin 5 mRNA and a 70.6% and 80.5% significant increase in the expression of Bax mRNA （P〈0.05, P〈0.01） in the rat testis on days 14 and 28 respectively. There was no significant change in the expressions of annexin 5 and Bax mRNA on day 42 compared with the control groups. Conclusion The removal of submandibular gland can significantly increase the expressions of annexin 5 and Bax mRNA in the rat testis on days 14 and 28. The expressions of annexin 5 and Bax mRNA goes back to the normal level on day 42.

关 键 词：下颌下腺 睾丸 ANNEXIN 5 BAX 荧光定量RT-PCR 大鼠 

分 类 号：R339.21[医药卫生—人体生理学]