血红素氧合酶-1对心肌相对缺血/再灌注损伤的延迟保护作用  

DELAYED PROTECTION OF HO-1 IN THE EXERCISE PRECONDITIONING FROM THE MYOCARDIAL RELATIVE ISCHEMIC REPERFUSION INJURY

作　　者：翟庆峰[1] 刘洪涛[2] 李媛媛[3] 马强[2] 

机构地区：[1]潍坊医学院,山东潍坊261042 [2]军事医学科学院卫生学环境医学研究所,天津300050 [3]潍坊市妇幼保健院,山东潍坊261011

出　　处：《中国应用生理学杂志》2008年第1期50-53,共4页Chinese Journal of Applied Physiology

摘　　要：目的:探讨血红素氧合酶-1(HO-1)在运动预适应(EP)中对大鼠心肌相对缺血/再灌注(rI/R)损伤的延迟保护作用。方法:40只Wistar大鼠随机分为5组:正常对照组(CN)、相对缺血/再灌注组(IR)、运动预适应+相对缺血/再灌注组(EI)、Hemin(HO-1诱导剂)+相对缺血/再灌注组(HE)和运动预适应+ZnPP(HO-1抑制剂)+相对缺血/再灌注组(EZ)。测定大鼠再灌注期心率脉压乘积(PRP)、冠脉流出液MDA含量、HO-1活性等。结果:心肌HO-1活性:EI组和HE组较IR组显著升高,EZ组则显著降低。EZ组较EI组显著降低,EI组和HE组间亦有显著性差异。再灌注后60 min时间点PRP恢复率:EI组较IR组显著增高;IR组与HE组未见显著性差异,但30min时间点HE组显著增高;EZ组较EI组显著降低。冠脉流出液MDA含量:EI组、EZ组和HE组MDA含量较IR组皆显著降低;EZ组较EI组显著升高。结论:EP可以诱导HO-1合成,进而通过HO-1对24 h后发生的rI/R损伤产生延迟保护作用。Aim： To explore the role of HO-1 in the exercise preconditioning protecting the rat myocardium from the relative myocardial ischemia/reperfusion injury（rI/R）. Methods： 40 Wistar rats were divided into 5 groups randomly： control group（CN）, relative ischemia/reperfusion group（IR）, exercise preconditioning ＋ relative ischemia-reperfusion group（EI）, HO-1 revulsant group（HE） and exercise preconditioning ＋ HO-1 inhibitor（EZ）group. We detected the cardiac function parameter-pressure-rate product（PRP） , the levels of MDA in coronary effluent and the activity of HO-1. Results： The activities of HO-1 of EI group and HE group were higher significantly than the IR group. EZ group was lower than EI group. There was significant difference between EI group and HE group. The recovery rate of PRP in the 60 rain point of reperfusion of El group was higher significantly than IR group, EZ group was lower than EI group. There was significant difference between HE group and IR group in 30 rain point of reperfusion. The levels of MDA in coronary effluent after rI/R of EI group, EZ group and HE group were lower significantly than IR group. There was significant difference between EI group and EZ group. Conclusion. EP can activate the HO-1 which can ameliorate the rI/R injury occurred after 24 hours.

关 键 词：血红素氧合酶-1 运动预适应 延迟预适应 缺血/再灌注 

分 类 号：R363[医药卫生—病理学]