人参皂苷Rg1组合诱导人成骨肉瘤MG-63细胞分化过程中Prohibitin的表达与定位变化  

Differentiation Induced by Ginsenoside Rg1 Combined Formula Involved the Alteration of Prohibitin Localization and Expression in Human Osteosarcoma MG-63 Cells

作　　者：石松林[1] 李祺福[1] 刘庆榕[1] 许东辉[2] 唐剑[1] 梁盈[1] 

机构地区：[1]厦门大学生命科学学院,细胞生物学和肿瘤细胞工程教育部重点实验室,福建厦门361005 [2]福建医科大学附属厦门第一医院肝、胆、胰、血管外科普外科,福建厦门361005

出　　处：《中国生物化学与分子生物学报》2008年第2期180-187,共8页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金资助项目(No.30470877)~~

摘　　要：选择性抽提经人参皂苷Rg1组合(RCT)诱导处理前后的人成骨肉瘤MG-63细胞核基质,对prohibitin在核基质中的存在、分布及其与相关基因产物在RCT处理前后MG-63细胞中的共定位关系进行观察研究.蛋白质组学分析结果显示,prohibitin存在于人成骨肉瘤MG-63细胞核基质蛋白组分中,并在RCT处理后细胞核基质中表达下调;蛋白质印迹杂交确证了prohibitin在MG-63细胞核基质中的存在及其在RCT处理后下调变化;免疫荧光显微镜观察进一步证实prohibitin定位在核基质上,经RCT处理后出现分布位置与表达水平变化;激光共聚焦显微镜观察可见prohibitin与c-Fos、c-Myc、p53和Rb基因产物均存在共定位关系,并在RCT处理后共定位分布区域出现变化.本研究证实了prohibitin是一种新发现的核基质蛋白,其在核基质上的定位与表达在RCT诱导分化前后发生显著变化,并与相关癌基因、抑癌基因产物存在共定位关系.实验表明RCT处理引起的prohibitin的变化与人成骨肉瘤MG-63细胞的诱导分化与调控具有密切关系,为深入揭示RCT等中药有效成分诱导肿瘤细胞分化的机理提供了重要科学依据和深入探索的新方向.Prohibitin is a nuclear matrix protein and associates with various oncogene products. A combined formula with ginsenoside Rgl, cinnamic acid and tanshinone Ⅱ A （RCT） was used to treat human osteosarcoma MG-63 cells in this study. We found that the prohibitin expression in the nuclear matrix was of decreased following RCT treatment by Western blot. The colocalization of prohibitin with the products of oncogenes or tumor suppressor genes, including c-Fos, c-myc, p53 and Rb was evaluated by immunofluorescence staining and laser scanning confocal microscopy. We observed significant alternations of prohibiitin distribution and the degree of colocalization with the associated gene products after the cells exposed to RCT. The results suggested that prohibitin might be involved in the RCT-induced differentiation in MG-63 ceils. Our findings may provide information to expand the applications of Traditional Chinese Medicine formulas in cancer-related studies.

关 键 词：PROHIBITIN 核基质 人成骨肉瘤MG-63细胞 细胞分化 人参皂苷RG1 

分 类 号：Q245[生物学—细胞生物学] Q254