糖皮质激素受体基因ER22/23EK多态性与新疆维吾尔族人自然长寿的关系及与汉族人群的比较  

Association of ER22/23EK polymorphism in glucocorticoid receptor gene with natural longevity in Xinjiang Uygur nationality and comparison with Han nationality

作　　者：姜文锡[1] 程祖亨[1] 牛文全[2] 邱长春[2] 

机构地区：[1]新疆医科大学,新疆乌鲁木齐830054 [2]中国医学科学院中国协和医科大学基础医学研究所医学分子生物学国家重点实验室,北京100005

出　　处：《基础医学与临床》2008年第2期113-118,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(30160089);美国中华医学基金(CMB96-657)

摘　　要：目的 探讨糖皮质激素受体基因（GR）第2外显子ER22／23EK多态性与维吾尔族人自然长寿的关系及种族差异。方法选择新疆维吾尔族191名年龄〉90岁的健康个体为长寿组，另选53名年龄（65-70岁）、性别、地域相匹配已自然死亡的正常个体为对照组；同时随机调查移居新疆和田地区30年以上的（65-70岁）汉族老年人82人。采用序列特异引物PCR（PCR-SSP）、PCR-限制性片段长度多态性（PCR-RELP）和PCR-直接测序（PCR-sequeneing）等技术对GR基因进行分型。结果191名自然长寿老人和53名对照组中，ER22／23EK的等位基因和基因型频率无差异。新疆汉族老人GR基因ER22／23EK携带者显著高于维吾尔族老人，其WM、MM基因型（P〈0．001）和M等位基因频率（P〈0．0001）均显著高于维吾尔族老人，而册基因型和形等位基因频率则显著降低。结论ER22／23EK的等位基因和基因型频率可能与维吾尔族自然长寿无关联，但有显著的种族差异。Objective To investigate association of the ER22/23EK polymorphism of the glucocorticoid receptor gene （GR） with natural longevity in the XinJiang Uygur nationality people and the race difference. Methods One hundred and nintyone healthy individuals over 90 years old from Uighur people were recruited as the longevity group at the same time, 82 Han nationality people aged between 65 - 70 who immigrated in Xinjiang Hetian for more than 30 years were randomly selected and investigated. Genotyping was performed by PCR-SSP, PCR-RELP and PCR sequencing. Results The frequencies of ER22/23EK alleles and genotypes showed no significant difference between the longevity group and the controls in Uygur, but the carriers of ER22/23EK of GR gene in Xinjiang Han old folks were significantly more than those in Uygurs, the frequencies of WM, MM genotypes and M allele were significantly higher in Han nationality, while the frequencies of WW genotype and W allele were significant lower. There is possibly no association between ER22/23EK polymorphism and XinJiang Uygur natural longevity, but there are significant differences between the two ethnic groups.

关 键 词：糖皮质激素受体基因 自然长寿 维吾尔族 汉族 

分 类 号：R394[医药卫生—医学遗传学]