食管癌患者指甲的红外光谱研究  

Study on FTIR Spectra of Finger Nails of Normal People and Patients of Esophagus Cancer

作　　者：汪红艳[1] 吕银[1] 王凡[1] 马晓冬[2] 蒋诗平[2] 王卫[2] 李承祥[2] 

机构地区：[1]安徽医科大学第一附属医院肿瘤放疗科,安徽合肥230022 [2]中国科学技术大学国家同步辐射实验室,安徽合肥230029

出　　处：《光谱学与光谱分析》2008年第2期331-334,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(60477001);安徽省自然科学基金项目(2003KJ218)资助

摘　　要：利用傅里叶红外光谱对正常人,食管癌患者,食管癌术后5个月者的指甲进行了研究。发现他们指甲的红外光谱在频率,强度,峰形等方面均有差异。主要表现在:食管癌患者νs(PO2-)和νas(PO2-)的振动峰均向高波数偏移,术后者回落;癌患者蛋白质C—O基团峰强度增强,术后者峰强度有所减弱,但仍比正常人强;癌患者角蛋白脂质CH2和CH3伸缩振动峰波数有所下降。术后者介于癌患者与正常人之间;食管癌患者角蛋白脂质CH2和CH3弯曲振动峰稍向高波数偏移,术后者恢复至正常人水平;癌患者酰氨Ⅰ带和Ⅱ带都向低波数偏移,术后者介于两者之间。这些差异是应用红外光谱诊断食管癌的基础,表明FTIR光谱在食管癌的早期诊断和快速诊断方面有着十分美好的前景。To investigate the application of human finger nails in the diagnosis of cancer, Fourier transform infrared （FTIR） spectroscopy was employed to study the finger nails from some normal people and some with esophagus cancer and others with an operation for curing esophagus cancer five months ago. The results showed that there are obvious differences between FTIR spectra in them in spectral parameters such as frequency, intensity and band shape etc. The changes in the phosphate symmetric stretching vibration vs （PO2^-） and asymmetric stretching vibration vas（PO2^- ） modes are uniform, the vs （PO2^-） and vss（PO2^- ） absorption peaks of cancerous ones shift to high wave number compared with those of normal people, while those with operation shift to low wave number compared with those of cancerous ones. The C--O stretching vibration mode of protein located at 1 164 cm^-1 is composed of three absorption peaks located at 1 173. 3, 1 158.0 and 1 151.1 cm^-1 respectively, meanwhile, the intensities and the wave numbers of the three peaks of cancerous ones all increase compared with normal people. The wave numbers of amide Ⅰ and amide Ⅱ of cancerous ones are both lower than those of normal people, while those with operation are between the cancerous ones and normal people, which suggest that the contents of protein and α-helix in finger nails of normal people, cancerous ones and the ones with operation are discriminative. The peak of bending vibration δ（CH2 ）mode of CH2 groups of protein lipid of cancerous ones shifts to high wave number slightly and the intensity of the peak weakens compared with that of normal people, which indicate that the methylene chain in the finger nails membrane lipids of cancerous ones is more ordered than that of normal people. Nevertheless, the peak of stretching vibration vs （CH2）of cancerous ones is lower than that of normal people, while that of the ones with operation is between cancerous ones and normal ones. As a result, the pathological changes in viscer

关 键 词：食管癌 傅里叶变换红外光谱 指甲 

分 类 号：R730.4[医药卫生—肿瘤] R735.1[医药卫生—临床医学]