HIV-1 C/B’亚型多价重组MVA病毒疫苗免疫原性的研究  

Immunogenicity in a Prime-boost Regimen of a Modified Vaccinia Virus Ankara (MVA) Vectored Experimental HIV-1 Clade C/B’ Vaccine

作　　者：高燕[1] 孙明[1] 蒋国润[1] 张银川[1] 王玺[1] 李冰香[1] 黄小琴[1] 陈丹[1] 周东霞[1] 褚嘉祐[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,昆明650118

出　　处：《中国生物工程杂志》2008年第2期11-15,共5页China Biotechnology

基　　金：国家"973"计划资助项目(2006CB504200)

摘　　要：目的:检测人类免疫缺陷病毒1型(HIV-1)C/B’亚型与痘苗病毒安卡拉株(MVA)的多价重组疫苗的免疫原性,此疫苗中包含HIV-1C/B’CRF株的5个基因,分别是env,gag,pol,nef,tat。方法:设105pfu/ml,106pfu/ml,107pfu/mlADMVA3个剂量组,用ELISPOT方法检测细胞免疫反应。同时,以Gag蛋白作为包被抗原,使用间接ELISA的方法检测体液免疫反应。结果:免疫后的小鼠对插入基因env,gag,pol和nef所表达蛋白均产生了特异性细胞免疫应答,且免疫效果与免疫剂量呈正相关。ELISA结果表明,MVA重组疫苗免疫诱导产生了特异性体液免疫,抗HIV-1Gag的抗体滴度与免疫次数和免疫剂量都存在正相关性。结论:多价MVA重组疫苗能有效地诱导小鼠产生特异的细胞免疫和体液免疫反应。Objective： To evaluate the immunogenicity of HIV-1 clade C/B＇ vaccine based on modified vaccinia virus Ankara （MVA） vector in mice. Methods： Mice were inoculated with 3-dose HIV vaccine by intramuscular injection. Blood sample were collected every second week, and then the antibodies against HIV were detected. At week 6, mice were killed and cellular immune responses were examined by ELISPOT. Result ： The number of spot forming cells in the 107 pfu/ml -dose group was more than those of 10^5 pfu/ml -dose and 10^6 pfu/ml -dose groups significantly. HIV specific antibodies emerged at week 2 and elevated rapidly at week 4 and week 6. The level of specific IgG in the 107 pfu/ml -dose group was more than those of 10^5 pfu/ml -dose and 106 pfu/ml -dose groups significantly. Conclusion： The ADMVA induces both humoral immunoresponse and cellular immune responses.

关 键 词：HIV-1 C/B’亚型 多价重组MVA病毒疫苗 免疫原性 

分 类 号：Q819[生物学—生物工程]