小麦-黑麦二体异附加系分子细胞遗传学鉴定  

Molecular Cytogenetics Identification of Wheat-Rye Alien Disomic Additional Line

作　　者：吴金华[1] 王长有[1] 王秋英[1] 吉万全[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨陵712100

出　　处：《西北植物学报》2008年第1期59-64,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家科技支撑计划"小麦育种"(2006BAD01A02);小麦种质资源繁种更新与利用[NB07-2130135-(25-30-0301)];陕西省重大专项(2006kz05-G3)

摘　　要：用形态学、细胞学、生化及分子标记方法,对小麦-黑麦衍生系N9436-0-2-29-9进行分析鉴定,以明确N9436-0-2-29-9中具体的外源染色体。农艺性状调查表明,N9436-0-2-29-9衍生系在形态上整齐一致,对白粉病高抗;细胞学分析表明,染色体构型稳定,2n=44=22Ⅱ;Giemsa C-分带和SSR分析表明,该衍生系为普通小麦-黑麦6R二体异附加系;高分子量麦谷蛋白亚基分析(HMW-GS)表明,外源染色体的导入使该衍生系的高分子量麦谷蛋白亚基表达受到影响。In order to define the chromosome from rye in N9436-0-2-29-9, wheat-rye derivative line was identified by morphological, cytological, biochemical and molecular marker method. It was showed in agronomic characters that it was stable morphologically and high resistant to powdery mildew. By analysis of cytolo- gy,it was displayed that its configuration was 2n=44=22 Ⅱ. Through Giemsa C-banding and simple sequence repeat（SSR）,this line was identified 6R alien disomie additional line. Finally by high＇molecularweight glutenin subunit （HMW-GS）, it was analyzed that introduction of alien chromosome affected the composition of HMW-GS.

关 键 词：奥地利黑麦 二体异附加系 GIEMSA C-分带 SSR分析 高分子量麦谷蛋白亚基分析 

分 类 号：Q343.22[生物学—遗传学]