微弧氧化处理对钛合金TLM表面成骨细胞形态及整合素β1表达水平的影响  

Effects of micro-arc oxidation on morphology and integrin β1 expression of osteoblasts cultured on Ti-5Zr-3Sn-5Mo-15Nb titanium alloy.

作　　者：赵领洲[1] 张玉梅[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《临床口腔医学杂志》2008年第1期12-15,共4页Journal of Clinical Stomatology

基　　金：国家高技术研究发展计划(863计划)资助(2002AA326070)

摘　　要：目的:采用微弧氧化(MAO)方法对近β钛合金Ti-5Zr-3Sn-5Mo-15Nb(TLM)进行表面处理,观察其对成骨细胞在其表面粘附形态及整合素表达水平的影响。方法:用脉冲直流电源在两个不同电压下对TLM表面进行微弧氧化处理。原代大鼠头盖骨成骨细胞在试样表面培养72h后电镜观察其形态。RT-PCR方法检测细胞培养24h、72h和120h后整合素β1表达。结果:成骨细胞在对照组表面呈单层生长,细胞沿抛光纹路排列并伸出大量丝状和板状伪足。在微弧氧化TLM表面,成骨细胞生长呈双层网状结构,较对照组表面伸出更多的板状伪足。微弧氧化TLM表面成骨细胞的伸展较对照组好,尤其是高电压微弧氧化表面。RT-PCR结果显示观察时间内整合素β1的表达水平并未受微弧氧化处理的影响。结论:微弧氧化TLM表面成骨细胞的粘附和伸展优于对照组表面,但此过程中整合素β1的表达水平在各个组间并无差异,推测微弧氧化表面可能是通过影响整合素β1的构型有利于其活化,进而促进成骨细胞粘附和伸展。Objective： This study was intended to modify the surface of a novel near β titanium alloy Ti-5Zr-3Sn- 5Mo-15Nb （TLM） by micro-arc oxidation （MAO）, and evaluate the influence of MAO process on the morphology and integrin β1 （Itgβ1） expression of osteoblasts cultured on TLM. Method： MAO of the specimens was carried out using a pulsed DC power supply at two different voltages. SEM was used to observe the morphology of osteoblasts cultured on different TLM surfaces for 72 h. RT-PCR was used to analyze the Itgβ1 expression of osteoblasts on TLM surfaces for 24,72 and 120 h. Result： On the polished surface,the osteoblasts exhibited monolayer proliferation with large amount of fillopodia and lamellipodia, Most of which showed an elongated shape and aligned along the direction of the grooves. While on the microarc oxidized surfaces,the osteoblasts formed a double layer net-like structure, with a greater number of lamellipodia coming out from the cells. It seemed that the osteoblasts spread better than those on the polished surface, especially on the surface formed at high voltage. No variation in the Itgβ1 expression of osteoblasts was observed with respect to different surfaces. Conclusion： Osteoblasts adhesion and spread are better on MAO surfaces than on the control surface, while no obvious difference of Itgβ1 expression is found on different surfaces. It＇s supposed that MAO surfaces benefit the activation of Itgβ1 via conformation change,thus enhance osteoblasts adhesion and spread.

关 键 词：近Β钛合金 微弧氧化 成骨细胞 粘附 整合素 

分 类 号：Q786[生物学—分子生物学] R783.1[医药卫生—口腔医学]