核因子κB圈套策略对膀胱癌细胞侵袭力的影响  

Effect of circular dumbbell oligodeoxynucleotides of nuclear factor kappa B on the invasion ability of bladder cancer cell

作　　者：文博[1] 周四维[2] 杨为民[2] 宋晓东[2] 李国颢[3] 刘征[2] 

机构地区：[1]深圳市宝安区人民医院泌尿外科,518101 [2]华中科技大学同济医学院附属同济医院泌尿外科 [3]武汉市中心医院

出　　处：《中华实验外科杂志》2008年第2期245-247,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30271300）

摘　　要：目的探讨核因子（NF）-κB环状哑铃型圈套寡核苷酸（CD—ODN）对膀胱癌NF—κB活性和细胞侵袭能力的影响以及有关的作用机制。方法脂质体介导法瞬时转染CD—ODN及其突变体（CD-ODN-mut）入膀胱癌BIU87细胞株，荧光素酶报告基因的方法检测不同处理组NF—κB活性的改变；逆转录．聚合酶链反应（RT-PCR）、Western—blot检测尿激酶型纤溶酶原激活剂（uPA）的表达水平；重建基底膜试验检测细胞侵袭能力的变化。结果转染CD-ODN后BIU87细胞株NF—κB活性被抑制，uPA的表达水平下降，细胞侵袭能力减弱，而转染CD—ODN—mut后无此作用。结论NF-κB组成性活化可促进uPA的表达，从而导致膀胱癌细胞的侵袭和转移。而CD—ODN能有效的阻断这一通路，降低膀胱癌的侵袭潜能。Objective To investigate the effect of circular dumbbell DNA oligodeoxynucleotides （CD-ODN） on NF-κB activity,cell invasion ability and the related molecule mechanism in bladder cancer cell line BIU87. Methods CD-ODN and CD-ODN-mut were transfected into human bladder cancer cell line BIU87 by Lipofectamine. Luciferase reporter was applied for detecting NF-κB DNA binding activity. The expression levels of uPA were detected by RT-PCR and Western blot, and the invasion ability by Transwell cell culture chamber. Results After transfection of CD-ODN into BIU87 cells, the activity of NF-κB was decreased significantly,the invasion ability weakened obviously and the uPA expression was inhibited as well. Conclusion NF-κB is constitutively activated in bladder cancer,which suggested that NF-κB activity is related to tumor progression due to its transcriptional regulation of uPA. CD-ODN can effectively inhibit the DNA binding activity of NF-κB in vitro and reduce the invasion ability of tumor.

关 键 词：膀胱癌 核因子ΚB 寡核苷酸素 

分 类 号：R737.14[医药卫生—肿瘤]