茶多酚对大鼠肠缺血再灌注肠损伤的保护作用  

Protective effects of tea polyphenols on intestinal injury induced by intestinal ischemia reperfusion in rats

作　　者：王利[1] 吕莉[2] 韩国柱[2] 李楠[3] 

机构地区：[1]大连市友谊医院普外科,大连116001 [2]大连医科大学药理教研室,大连116027 [3]大连理工大学分析化学教研室,大连116023

出　　处：《中国新药杂志》2008年第4期296-299,302,共5页Chinese Journal of New Drugs

摘　　要：目的:研究茶多酚(TP)对大鼠肠缺血再灌注(I/R)所致肠损伤的保护作用及其可能的作用机制,为其临床新用途提供实验依据。方法:大鼠随机分为肠I/R损伤对照组、假手术组及TP给药组(100,50,25和12.5 mg.kg-1),通过夹闭肠系膜上动脉1 h、再灌注2 h,建立肠I/R损伤模型。于缺血前20 m in舌下静脉注射药物,假手术组仅分离、不夹闭肠系膜上动脉。再灌2 h后,各组取血及中段小肠组织,测定血清及小肠组织中超氧化物歧化酶(SOD)、丙二醛(MDA)和一氧化氮(NO)含量,光镜下观察小肠组织形态学改变。结果:与假手术组相比,肠I/R损伤对照组血清及小肠组织中的SOD活力降低,MDA和NO含量升高,镜检发现小肠有明显组织形态学损伤。与肠I/R损伤对照组相比,TP组呈剂量依赖性增强SOD活力,减少MDA及NO含量,减轻小肠组织形态学损伤。结论:TP对肠I/R所致肠急性损伤有显著的及剂量依赖性的保护作用,可能与其自由基清除作用有关。Objective： To investigate the protective effects of tea polyphenols （TP） on intestinal injury induced by intestinal ischemia/reperfusion （I/R） and the possible mechanism, to provide pharmacological evidence for its new clinical uses. Methods： Rats were randomly divided into six groups： the sham group, I/R injury control group,and TP groups （100,50,25 and 12.5 mg·kg^-5）. The intestinal I/R injury was induced by clamping superior mesenteric artery for 1 h and reperfusing for 2 h. TP was administered by sublingual vein at 20 min before ischemia. Blood samples and mid-segment intestine samples were collected 2 h after reperfusion. SOD activity, MDA and NO contents in serum and intestine were determined,and pathological changes of intestine were observed. Resuits： After intestinal I/R injury, activity of SOD was decreased, while the levels of MDA and NO were increased in serum and intestine. In addition, intestine was destroyed obviously. TP dose-dependently increased SOD activity, decreased MDA and NO content in both serum and intestine, and attenuated pathological changes in intestine tissue after I/R injury. Conclusion： TP can dose-dependently protect against intestinal I/R injury. This protective effect relates to its anti-oxidant action.

关 键 词：茶多酚 肠缺血再灌注 超氧化物歧化酶 丙二醛 一氧化氮 

分 类 号：R965.1[医药卫生—药理学] R977.9[医药卫生—药学]