椒目中α-亚麻酸的提取与鉴定  

Study on extraction and identification of α-linoleic acid from Semen Zanthoxyli Bungeani

作　　者：杨倩[1] 王四旺[1] 王剑波[1] 谢艳华[1] 刘喆[1] 孙纪元[1] 缪珊[1] 苗青[1] 

机构地区：[1]第四军医大学药学系药物研究所,西安710033

出　　处：《中国新药杂志》2008年第4期318-320,共3页Chinese Journal of New Drugs

基　　金：陕西省中医药管理局资助项目(2005079)

摘　　要：目的：从花椒的种子即椒目中分离和纯化α-亚麻酸,鉴定化学结构并进行含量测定。方法：椒目粗粉采用皂化、酸化、梯度冷冻、尿素包合及硝酸银络合等步骤分离和纯化不饱和脂肪酸,并用HPLC测定α-亚麻酸含量,鉴定结构。结果：提取样品中α-亚麻酸的含量为91.6%-95.2%;结构为9,12,15-十八碳三烯酸（z,z,z-9,12,15-octadecatrienoic acid）,全顺式,非共轭立体构型,分子式为C18H30O2。结论：本研究从废弃资源椒目中提取分离得到高纯度α-亚麻酸,因此,椒目是α-亚麻酸的良好资源。Objective ：To extract and purify α-linoleic geani,viz Semen Zanthoxyli Bungeani,to identify the chemical acid from the seeds of Pericarpium Zanthoxyli Bunconstitution and determine the contents. Methods： Semen Zanthoxyli Bungeani coarse powder was processed by saponification, acidification, gradient freezing, urea clathration and becoming argent nitrate complex. Finally,the highly purified α-linoleic acid was obtained. The contents were assayed by HPLC, and the structure was identified. Results：The content of α-linoleic acid was in the range of 91.6% - 95.2%. The structure was identified to be z,z,z-9,12,15 octadecatrienoic acid. The formula was C18H30O2. Conclusion：The pure α-linoleic acid was obtained from Semen Zanthoxyli Bungeani. Semen Zan- thoxyli Bungeani is a new resource of α-linoleic acid.

关 键 词：椒目 Α-亚麻酸 亚油酸 

分 类 号：R284.1[医药卫生—中药学] R344.7[医药卫生—中医学]