Non-invasive diagnosis of H pylori infection: Evaluation of serological tests with and without current infection marker CIM  

作　　者：Sufi HZ Rahman M Golam Azam M Anisur Rahman MS Arfin M Mahbub Alam Tareq M Bhuiyan Nasim Ahmed Motiur Rahman Shamsun Nahar MS Hassan 

机构地区：[1]Department of Immunology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders, Dhaka, Bangladesh [2]Department of Gastrointestinal Hepatobiliary and Pancreatic Diseases, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders, Dhaka, Bangladesh [3]Department of Pathology, Ibrahim MedicalCollege, Dhaka, Bangladesh [4]Laboratory Sciences Division, International Centre for Diarrhoeal Diseases Research, Bangladesh, Dhaka, Bangladesh

出　　处：《World Journal of Gastroenterology》2008年第8期1231-1236,共6页世界胃肠病学杂志（英文版）

基　　金：SIDA Grant, No. GR-00384 to MotiurRahman

摘　　要：AIM:To evaluate the performance of commercially available immunochromatographic (ICT) and immunoblot tests covering the current infection marker CIM and conventional ELISA for the diagnosis of H pylori infection in adult dyspeptic patients. METHODS:Consecutive non-treated dyspeptic patients undergoing diagnostic endoscopy were tested for H pylori infection by culture, rapid urease test, and histology of gastric biopsy specimens. Serum from 61 H pylori infected and 21 non-infected patients were tested for anti-H pylori IgG antibodies by commercial ELISA (AccuBindTM ELISA, Monobind, USA), ICT (Assure H pylori Rapid Test, Genelabs Diagnostics, Singapore), and immunoblot (Helico Blot 2.1, Genelabs Diagnostics, Singapore) assays. ICT and immunoblot kits cover CIM among other parameters and their performance with and without CIM was evaluated separately. RESULTS:Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of ELISA were 96.7%, 42.8%, 83.1%, 81.8%, and 82.9%, of ICT were 90.1%, 80.9%, 93.2%, 73.9%, and 87.8%, of ICT with CIM were 88.5%, 90.4%, 96.4%, 73.0%, and 89.0%, of immunoblot were 98.3%, 80.9%, 93.7%, 94.4%, and 93.9%, and of immunoblot with CIM were 98.3%, 90.4%, 96.7%, 95.0%, and 96.3%, respectively. CONCLUSION:Immunoblot with CIM had the best performance. ICT with CIM was found to be more specific and accurate than the conventional ELISA and may be useful for non-invasive diagnosis of H pylori infection.AIM： TO evaluate the performance of commercially available immunochromatographic （ICT） and immunoblot tests covering the current infection marker CIM and conventional ELISA for the diagnosis of Hpylori infection in adult dyspeptic patients.METHODS： Consecutive non-treated dyspeptic patients undergoing diagnostic endoscopy were tested for H pylori infection by culture, rapid urease test, and histology of gastric biopsy specimens. Serum from 61 H pylori infected and 21 non-infected patients were tested for anti-H pylori IgG antibodies by commercial ELISA （AccuBindTM ELISA, Monobind, USA）, ICT （Assure H pylori Rapid Test, Genelabs Diagnostics, Singapore）, and immunoblot （Helico Blot 2.1, Genelabs Diagnostics, Singapore） assays. ICT and immunoblot kits cover CIM among other parameters and their performance with and without CIM was evaluated separately.RESULTS： Sensitivity, specificity, positive predictive value （PPV）, negative predictive value （NPV）, and accuracy of ELISA were 96.7%, 42.8%, 83.1%, 81.8%, and 82.9%, of ICT were 90.1%, 80.9%, 93.2%, 73.9%, and 87.8%, of ICT with CIM were 88.5%, 90.4%, 96.4%, 73.0%, and 89.0%, of immunoblot were 98.3%, 80.9%, 93.7%, 94.4%, and 93.9%, and of immunoblot with CIM were 98.3%, 90.4%, 96.7%, 95.0%, and 96.3%, respectively.CONCLUSION： Immunoblot with CIM had the best performance, ICT with CIM was found to be more specific and accurate than the conventional ELISA and may be useful for non-invasive diagnosis of Hpylori infection.

关 键 词：H pylori ELISA Immunochromatographic test IMMUNOBLOT Current infection marker 

分 类 号：R573[医药卫生—消化系统]