含NURR1基因重组腺病毒旁分泌对神经干细胞分化的影响  

A Paracrine Effect of Adenovirus with NURR1 Gene (Ad-NURR1) on Differentiation of Neural Stem Cells

作　　者：李庆军[1] 肖颂华[1] 肖卫民[1] 江东新[1] 

机构地区：[1]东莞市人民医院神经内科,广东东莞523018

出　　处：《神经损伤与功能重建》2008年第1期22-24,共3页Neural Injury and Functional Reconstruction

基　　金：广东省医学科学技术研究基金资助项目(资助项目WSTJJ20041213)

摘　　要：目的:探讨含NURR1基因的腺病毒(Ad-NURR1)旁分泌效应对C17.2神经干细胞分化的影响。方法:用Western blot检测Ad-NURR1 nurr1蛋白分泌,以旁分泌试验检测nurr1蛋白对神经干细胞分化的影响。结果:Western blot证实Ad-NURR1表达的nurr1蛋白可分泌到细胞外上清内,并可明显促进神经干细胞向神经元方向分化。结论:nurr1蛋白可分泌到细胞外并能促进神经干细胞分化。Objective..To study the paracrine effect of adenovirus with NURR1 gene （Ad-NURR1） on differentiation of C17.2 neural stem cells. Methods：Nurrl protein excreted by Ad-NURR1 was probed by Western blot- ting. Differentiation of C17.2 cells induced by Nurrl protein was tested by paracrine trials. Result： Nurrl protein could be secreted into supernatant of C17.2 cells to enhance them to differentiate into neurons. Conclusion： Nurrl protein can be excreted and could induce differentiation of C17.2 cells.

关 键 词：NURR1 腺病毒 神经干细胞 帕金森病 

分 类 号：R741[医药卫生—神经病学与精神病学] R742.5[医药卫生—临床医学]