PAI-1在大鼠胆汁淤积性肝纤维化形成中的动态变化  

Dynamic changes of plasminogen activator inhibitor-1 in the process of cholestatic liver fibrosis in rats

作　　者：安德明[1] 季光[1] 郑培永[1] 

机构地区：[1]上海中医药大学附属龙华医院消化内科,上海中医药大学脾胃病研究所,上海市200032

出　　处：《世界华人消化杂志》2008年第3期246-252,共7页World Chinese Journal of Digestology

基　　金：教育部新世纪优秀人才支持计划资助项目;No.NCET-07-0563;上海市教委重点学科资助项目;No.J50305~~

摘　　要：目的:探讨纤溶酶原激活物抑制剂1(PAI- 1)mRNA在胆汁淤积性肝纤维形成中的动态变化及其作用.方法:应用胆管结扎法复制胆汁淤积性肝纤维化动物模型,分为假手术组,模型组1wk、2wk、3wk、4wk,观察模型大鼠肝组织病理、纤维化程度、RT-PCR检测PAI-1mRNA的水平、ELISA法检测MMP-2和MMP-9含量的动态变化.结果:随胆管阻塞时间延长,胆管阻塞性肝纤维化大鼠纤维化程度逐渐增加,模型组1wk、2wk、3wk、4wk组PAI-1mRNA表达与假手术组相比,逐渐增强,有显著性差异(1.53±0.01,1.84±0.03,2.06±0.04,3.62±0.04 vs 0.72±0.02,P<0.01),而MMP-2和MMP-9表达先升高后下降.结论:PAI-1mRNA表达存在动态变化,其表达升高与胆汁淤积性肝纤维化形成和发展有关.AIM： To study the dynamic changes of plasminogen activator inhibitor 1 （PAI-1） mRNA in the process of cholestatic liver fibrosis in rats. METHODS： Rat models of cholestatic liver fibrosis were induced by bile duct ligation. Rats were divided into control group, I wk, 2 wk, 3 wk and 4 wk groups. Liver pathology and fibrosis degree were observed. Expression of PAI-1 mRNA, MMP-2 and MMP-9 was detected weekly by ELISA. RESULTS： Histological examination showed that the degree of rat liver fibrosis and the expression of PAI-1 mRNA were gradually increased with the prolongation of biliary obstruction. The expression of PAI-1/β-actin mRNA in 1 wk, 2 wk, 3 wk and 4 wk model groups was significantly higher than that in the control group （1.53 ± 0.01, 1.84 ±0.03, 2.06 ± 0.04, 3.62 ± 0.04 vs 0.72 ± 0.02, P 〈 0.01） and the expression of MMP-2 and MMP-9 was elevated in the first stage and then declined. CONCLUSION： Dynamic changes of PAI-1 mRNA expression are closely correlated with the formation and development of cholestatic liver fibrosis.

关 键 词：I型纤溶酶原激活物抑制剂 胆汁淤积性肝纤维化 基质金属蛋白酶 逆转录聚合酶链式反应 

分 类 号：R575.2[医药卫生—消化系统] R575.105[医药卫生—内科学]