克隆与健脾益气法有关的大鼠肝癌新基因  

Cloning of novel hepatoma gene in rats by invigorating the spleen to supplement Qi

作　　者：管冬元[1] 方肇勤[1] 朱希[1] 吴中华[1] 张辉[1] 

机构地区：[1]上海中医药大学基础医学院,上海市201203

出　　处：《世界华人消化杂志》2008年第3期265-271,共7页World Chinese Journal of Digestology

基　　金：国家自然基金;No.30400582;No.30271576;上海市教委资助项目.No.04CB04~~

摘　　要：目的:克隆健脾益气法明显下调基因中EST片段cDNA的完整表达序列.方法:从689个健脾益气法明显下调的基因中,采用电子克隆与PCR相结合的方法,克隆出有关EST片段的cDNA完整表达序列.结果:成功筛选出11个EST片段的cDNA序列(G2、G4、G5、G6、G11、G14、G15、G16、G17、G18、G20).经进一步BLAST对比分析,证明G14、G15、G203个基因为新基因,并进行GenBank登录,登录号分别为DQ480745、DQ480746、DQ480747.结论:本研究成功克隆出3个中医健脾益气法能明显调控的新基因cDNA序列.这为今后进一步深入观察这些基因的功能以及探讨不同中医治法的作用机制提供了良好的研究基础.AIM： To clone the integrated cDNA expression sequence of EST segments in down-regulated genes by invigorating the spleen to supplement Qi. METHODS： Integrated cDNA expression sequences of EST gene segments were cloned for the 689 down-regulated genes by invigorating the spleen to supplement Qi, with electron cloning in combination with PCR. RESULTS： cDNA expression sequences were detected in 11 EST segments （G2, G4, G5, G6, Gll, G14, G15, G16, G17, G18, G20）. BLAST analysis showed that G14, G15 and G20 were novel genes which were submitted to GenBank （their accession number is DQ480745, DQ480746 and DQ480747, respectively）. CONCLUSION： Invigorating the spleen to supplement Qi can clone the cDNA expression sequences of EST segments in the down-regulated genes. Further study is needed to observe the functions of these novel genes and the mechanism of action of different TCM therapies.

关 键 词：健脾益气 肝癌 基因克隆 生物信息学 

分 类 号：R735.7[医药卫生—肿瘤]