甲基叔丁基醚对大鼠小脑急性毒作用机制  

Acute toxic mechanism of methyl tertiary butyl ether in rat cerebellum

作　　者：李素云[1] 丁红梅[1] 周翠兰[1] 熊伟[1] 周小兵[1] 贺性鹏[2] 

机构地区：[1]南华大学医学院解剖学教研室,衡阳421001 [2]南华大学公共卫生学院,衡阳421001

出　　处：《解剖学杂志》2008年第1期64-67,共4页Chinese Journal of Anatomy

基　　金：湖南省卫生厅科研项目基金(B2005090)

摘　　要：目的:观察甲基叔丁基醚(MTBE)对大鼠小脑Fos蛋白、γ-氨基丁酸(GABA)、ATP酶的影响,探讨MTBE对小脑急性毒作用机制。方法:72只健康成年SD大鼠,腹腔注射MTBE染毒,染毒剂量为0、700、1050、1400mg·kg-1,在各染毒剂量及染毒后0·5、1·0、2·0、4·0、8·0h时,用免疫组织化学方法检测MTBE对大鼠小脑Fos蛋白、GABA的表达并用相对灰度值定量;用分光光度法测定ATP酶的活性。结果:与对照组比较,小脑皮质Fos蛋白、GABA在MTBE染毒后0·5h时表达增加,1·0h达到高峰,2·0h时减弱,8·0h时恢复至正常;Na+,K+-ATP酶、Ca2+-ATP酶的活性染毒后1·0h时降低,染毒后8·0h时其活性无变化。结论:MTBE可作用于小脑,其急性毒作用可能与神经递质GABA含量的改变有关;MTBE可降低大鼠小脑Na+,K+-ATP酶、Ca2+-ATP酶的活性,改变神经细胞能量代谢。Objective： To explore the acute toxic mechanism of methyl tertiary butyl ether （MTBE） by detecting the changes of Fos protein, neurotransmitter 7-amino-butyric acid （GABA） and the activity of Na^＋ , K^＋ -ATPase, Ca^2＋ -ATPase in the cerebellar cortex. Methods： 72 adult SD rats were randomly divided into control and experiment groups. The MTBE exposed dose was 0, 700, 1 050 and 1 400 mg· kg^-1 and administrated by intraperitoneal injection. The immunohistochemical method （SABC method） was used to detect the change of Fos protein and neurotransmitter GABA in the cerebellar cortex. The spectrophotometric method was used to measure the activity change of the Na^＋ , K^＋ -ATPase, Ca^2＋ -ATPase in the cerebellar cortex. Results： The expression of Fos protein and GABA in cerebellar cortex changed in different time （0.5, 1.0, 2. 0, 4. 0, 8. 0 h） after being treated with MTBE （1 050 mg · kg^-1 ）. The expression of Fos protein and GABA began to increase at 0.5 h, reached peak at 1.0 h, and reduced at 2.0 h, and were recovered to normal at 8.0 h. The effect of MTBE on the activity of Na^＋ , K^＋ -ATPase, Ca^2＋ -ATPase in the cerebellar cortex was decreased dramatically in 1.0 h compared with the control （P〈0.05）. The activities of Na^＋ , K^＋ -ATPase, Ca^2＋ -ATPase were recovered to normal （P〉0.05） at 8.0 h. Conclusion： （1）MTBE could arise in the acute damage of the cerebellar cortex, which is relevant to the neurotransmitter GABA. （2）MTBE can reduce the activities of the Na^＋ , K^＋ -ATPase, Ca^2＋ -ATPase to affect the energy metabolism of the nerve cells.

关 键 词：甲基叔丁基醚 小脑皮质 FOS蛋白 γ-氨基丁酸 Na^+ K^+ - ATP酶 Ca^2+ -ATP酶 

分 类 号：R363[医药卫生—病理学]