碱性成纤维细胞生长因子调节脑缺血再灌注大鼠海马及顶叶皮质神经元β-Catenin的表达  

bFGF regulates expression of β-catenin in the neurons of hippocumpus and parietal cortex in cerebral ischemia and reperfusion rats

作　　者：邢雪松[1] 吕威力[2] 郭丽[3] 

机构地区：[1]沈阳医学院基础医学院解剖学教研室,沈阳110034 [2]沈阳医学院基础医学院病理学教研室,沈阳110034 [3]沈阳医学院基础医学院中心实验室,沈阳110034

出　　处：《解剖学杂志》2008年第1期74-76,共3页Chinese Journal of Anatomy

基　　金：辽宁省教育厅科学研究计划(05L442)

摘　　要：目的:探讨碱性成纤维细胞生长因子(bFGF)对脑组织缺血再灌注神经元β-Catenin的调节作用及机制。方法:应用线栓法制作大鼠局灶性脑缺血再灌注模型,大脑中动脉阻塞1h再灌注损伤24h,采用TUNEL法、免疫组织化学SABC法及显微图像分析检测海马及顶叶皮质内神经元凋亡和β-Catenin的表达。结果:大鼠缺血再灌注海马及顶叶皮质内神经元凋亡数增加而β-Catenin免疫阳性反应产物较正常组增加,注射bFGF后神经元凋亡数减少,β-Catenin免疫阳性反应产物明显多于缺血再灌注组。结论:bFGF抑制缺血神经元凋亡,参与β-Catenin的调节,对缺血神经元有保护作用。Objective： To investigate the expression of β-catenin in hippocampus after cerebral ischemia and reperfusion in rats, and explore the regulative effect and mechanism of basic fibroblast growth factor （bFGF） to β-catenin on brain tissue. Methods： The model of middle cerebral arteries occlusion （MCAO） was performed with intraluminal filament method. The expression of β-catenin and apoptosis cell in the hippocampus and parietal cortex were detected with SABC immunohistochemical method and TUNEL method, and analyzed by microirnage system. Results： The expression of β-catenin in hippocampus and cortex was increased after cerebral ischemia and reperfusion; the expression of β-catenin in bFCJF group was increased, but cell apoptosis was decreased. Conclusion： The results indicate that bFGF depresses cell apoptosis, participating in the regulation of the expression of β-catenin in the ischemic neurons.

关 键 词：脑缺血 碱性成纤维细胞生长因子 β联蛋白 海马 顶叶皮质 凋亡 

分 类 号：R743[医药卫生—神经病学与精神病学]