盐酸小檗碱作用于人舌癌Tca8113细胞的形态学研究  

Effect of inhibitor berberine of the morphology on Human tongue cancer Tca8113 cell pine

作　　者：刘杰[1] 曹选平[1] 薛晶[1] 

机构地区：[1]郑州大学口腔医学院,河南郑州450052

出　　处：《河南医学研究》2007年第4期317-319,共3页Henan Medical Research

摘　　要：目的:观察盐酸小檗碱作用与体外培养人舌癌Tca8113细胞的形态学改变,探讨盐酸小檗碱对Tca8113细胞的杀伤作用。方法:利用体外培养技术,HE染色法,丫啶橙染色法,观察盐酸小檗碱对Tca8113细胞作用后的形态学改变。结果:盐酸小檗碱(4μg/ml)作用于Tca8113细胞48h后,细胞形态发生改变,大量细胞变圆,细胞出现出芽现象,并出现较多的悬浮死亡细胞。结论:盐酸小檗碱可使Tca8113。细胞膜表面结构发生改变,并具有明显的杀伤作用。Objective： Observe the morphological changes, under the effects of berberine, of cultivated Human tongue cancer Tca8113 cells, and explore the killing effects of berberines on Tca8113. Methods： Adopt vitro cultivation techniques, He staining and acridine or ange staining to observe the morphological changes of Tca8113 cells under the effects of berberines. Results： After 48 hours, Tcas8113 cells shape changed, a large number of cells became round and budded, while there rose considerable number of suspended dead cells. Conclusion ： Berberines can change Tca8113 cells surface structure and show significant killing effects.

关 键 词：小檗碱 TEA8113细胞 丫啶橙 

分 类 号：R739.8[医药卫生—肿瘤]