星形细胞瘤细胞的原代培养及形态学研究  

Primary culture and morphology investigation of human astrocytoma

作　　者：孙力超[1] 孔令非[1] 赵跃武[1] 

机构地区：[1]河南省人民医院病理科,河南郑州450003

出　　处：《河南医学研究》2007年第4期320-323,共4页Henan Medical Research

基　　金：河南省自然科学基金项目(编号0611041700)

摘　　要：目的:通过建立人星形细胞瘤细胞原代培养的方法,观察不同级别星形细胞瘤细胞的生长状态及形态学特点。方法:应用胰蛋白酶消化法,对不同级别的新鲜星形细胞瘤手术标本进行肿瘤细胞原代培养及传代培养,通过调整培养参数,观察肿瘤细胞的生长情况,并从形态学及细胞免疫组化染色等方法进行研究。结果:Ⅱ级及以上的星形细胞瘤均成功进行原代细胞培养,Ⅲ及Ⅳ级星形细胞瘤还成功获得传代培养,并表达免疫组化GFAP,不表达Ⅳ胶原。结论:星形细胞瘤细胞的原代培养可以用胰酶消化法成功培养,培养显示,不同级别的星形细胞瘤其生长活性不同,与其恶性生物学行为呈密切相关。Objective： To establish the method of primary culture of astrocytoma cell in vitro and observe the different morphology between their histological grades. Methods： Using enzyme digesting method, astrocytes from the human astrocytoma tissues were cultured and identified by immunohistochemistry identification. Results ： Primary glioma were successfully cultured and immunohistochemistry analysis showed that the cells were stained positive for GFAP. Conclusion ： The method of digestion cultivation can successfully culture primary astrocytoma. Proliferation of cultured primary tumor cells can be an indicator of the tumous biological malignancy.

关 键 词：星形细胞瘤 原代培养 形态学 免疫组化 

分 类 号：R73[医药卫生—肿瘤]