化疗药物对原代胃癌细胞的体外杀伤效应及其与端粒酶逆转录酶mRNA表达的关系  

Anti-tumor effects of chemotherapeutic drugs on human gastric cancer cells in vitro and the relationship with expression of hTERT mRNA

作　　者：耿明[1] 尹迎春[1] 曹永成[1] 付质杰[1] 王兴友[1] 邰艳红[1] 

机构地区：[1]济南军区总医院病理科,250031

出　　处：《中华肿瘤杂志》2007年第11期838-841,共4页Chinese Journal of Oncology

摘　　要：目的观察化疗药物对原代胃癌细胞的体外杀伤效应,并探讨其与胃癌组织中端粒酶逆转录酶(hTERT)mRNA表达的关系。方法将68例新鲜胃癌组织制备单细胞悬液,台盼蓝染色计数活细胞,分别加入紫杉醇(TZX)、阿霉素(ADM)、顺铂(CDDP)、5-氟尿嘧啶(5-Fu)和丝裂霉素(MMC)培养48 h。四甲基偶氮唑盐(MTT)法观察药物作用后肿瘤细胞活力及代谢活性变化。用原位杂交技术检测hTERT mRNA的表达。结果不同个体的肿瘤细胞对化疗药物敏感性不同,与肿瘤分化有关,TAX、CDDP、5-Fu、ADM和MMC的胃癌细胞抑制率分别为(40.6±6.9)%、(38.4±7.8)%、(38.9±9.2)%、(31.6±8.5)%和(28.9±9.8)%。全组hTERT mRNA表达阳性率为90.0%,hTERT mRNA阳性者对5-Fu和ADM有较强的耐药性。结论hTERT mRNA的高表达可能是胃癌原发性耐药的标志之一,MTT比色法体外药敏实验有助于筛选胃癌的个体化有效化疗药物。Objective To evaluate in vitro antitumor effects of chemotherapeutic drugs, and investgate the relationship with expression of hTERT mRNA in human gastric cancer tissues. Methods Fresh samples of gastric cancer obtained from operation room were prepared to single-cell suspension （3 × 10^5 to 5 × 10^5 cells ml^-1） and were separately exposed to taxol （TAX）, cisplatin （CDDP）, 5-fluorouracil （5- Fu）, adriamycin （ADM）, mitomycin （MMC） for 48 hours. Metabolic activity and inhibitory rate of the cells were determined by trypan blue exclusion and MTT assay. Expression of hTERT mRNA was detected by in situ hybridization （ISH）. Results The inhibition rate of cancer cells exposed to chemotherapeutic drugs was different, and that of TAX, CDDP, 5-Fu was significantly higher than that of ADM and MMC. The positive rate of hTERT mRNA expression was 90.0% （54/60） and positive cells showed resistance to 5-Fu and ADM. Conclusion Overexpression of hTERT mRNA may contribute to primary drug-resistance of tumors. Chemosensitivity tests by MTT assay may contribute to prediction of effectness in applying chemotherapeutic drugs and identify drug-resistant cases.

关 键 词：胃肿瘤 HTERT mRNA 化疗药物敏感性 

分 类 号：R735.2[医药卫生—肿瘤]