饲粮营养水平对猪血液生化指标、背最长肌IMF含量及LPL mRNA表达量的影响  

Effects of dietary nutrition levels on blood biochemical parameters and IMF,LPL mRNA in Longissimus Dorsi of pigs

作　　者：廉红霞[1] 卢德勋[2] 高民[2] 

机构地区：[1]河南农业大学动物科学系,郑州市文化路95号450002 [2]内蒙古农牧业科学院动物营养所

出　　处：《中国饲料》2008年第5期12-15,20,共5页China Feed

基　　金：国家973重点基础研究发展计划“畜禽肉品质性状形成的营养代谢与调控机理”(2004CB117500)

摘　　要：试验选用84头体重接近10kg杜洛克×长白×大约克(DLY)三元杂交阉公猪,随机分成2组,组内设6个重复,每个重复7头猪。研究在NRC(1998)标准(对照组)、荣昌猪(GB7223-1987)饲养标准(试验组)两种饲粮条件下,营养水平对DLY生长肥育猪血液生化指标、背最长肌肌内脂肪(IMF)含量及LPLmRNA表达量的影响。结果表明:(1)对照组及试验组生长肥育猪血清中高密度脂蛋白胆固醇(HDL-C)含量、背最长肌肌内脂肪及LPLmRNA表达量含量随体重增加均呈上升趋势,35～80kg阶段血清游离脂肪酸(FFA)呈上升趋势,而后下降,而低密度脂蛋白胆固醇(LDL-C)与高密度脂蛋白胆固醇比值(LDL-C/HDL-C)呈下降趋势;(2)各阶段试验组背最长肌肌内脂肪含量均高于对照组,体重50kg时达到显著水平(P<0.05);体重110kg时,试验组背最长肌LPLmRNA表达量高于对照组(P>0.05);降低饲粮营养水平可降低35～110kg阶段血清胆固醇(TC)、HDL-C和FFA含量,降低了50～80kg阶段血清甘油三酯(TG)、LDL-C和极低密度脂蛋白胆固醇(VLDL-C)含量(P>0.05)。Eighty four healthy Duroc×Landrace ×Yorkshire pigs（ 10 kg BW） were allocated to control（NRC, 1998） or treatment group （GB 7223-1987）,each group with 6 replicates,each including 7 pigs,when body weight （BW） reached 110 kg, triglyceride （TG）, total cholesterol （TC）, high-density lipoprotein-cholesterol （HDL-C）, low-density lipoprotein- cholesterol （LDL-C）, very low-density lipoprotein-cholesterol （VLDL-C） and free fatty acid （FFA） in serum, intramuscular fat （IMF） and lipoprotein lipase （LPL） mRNA in Longissimus Dorsi were determined.The results showed that： （1）The concentration of serum HDL-C ,IMF and LPL mRNA in Longissimus Dorsi of control and treatment were increased when body weight （BW） increased during growing-finishing period.The concentration of serum FFA was increased when body weight was 35 - 80 kg,and decreased then.But the ratio of serum LDL-C/HDL-C had a tendency of decline when BW increased. （2）IMF in Longissimus Dorsi of treatment was higher than that of control during growing-finishing period ,and it was significant when BW was 50 kg（P 〈 O.05）.LPL mRNA in Longissimus Dorsi of treatment was higher than that of control when BW was 110 kg （P 〉 0.05）.As dietary nutritional level declined serum TC,HDL-C and FFA were decreased when BW was 35 - 110 kg,and serum TG,LDL-C and VLDL-C were also reduced when BW was 50 - 80 kg（P 〉 0.05）.

关 键 词：猪 LPL基因 肌内脂肪 生化指标 荧光定量RT—PCR 

分 类 号：S816.32[农业科学—饲料科学]