刺囊毛霉对甘草次酸的微生物转化研究  

Microbial transformation of glycyrrhetinic acid by Mucor spinous

作　　者：马晶[1] 辛秀兰[1] 张薇[1] 兰蓉[2] 刘俊英[2] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029 [2]北京电子科技职业学院生物技术应用中心,北京100029

出　　处：《北京化工大学学报（自然科学版）》2008年第1期79-82,共4页Journal of Beijing University of Chemical Technology(Natural Science Edition)

基　　金：北京市属市管高等学校人才强教计划

摘　　要：对刺囊毛霉AS3.3450微生物转化甘草次酸的条件进行了研究。结果表明,生成的主产物,经分析鉴定是7β-羟基甘草次酸;最佳转化条件为培养温度27℃,底物加量为80mg/L,转化10d,主产物得率为875mg/g甘草次酸。刺囊毛霉AS3.3450能够专一性地对甘草次酸进行羟基化反应,这为研究甘草次酸药物开发及体内代谢研究提供一定的理论依据。The major product of microbial transformation of glycyrrhetinic acid by Mucor Spinosus AS3.3450 has been identified as 7β-hydroxyglycyrrhetinic acid on the basis of its spectral data. The optimum conditions for the biotransformation found to be： incubation temperature of 27℃, substrate concentration of 80 mg/L, and incubation time of 10 d. The maximum transformation rate observed was 875 mg 7β-hydroxyglycyrrhetinic acid/g glycyrrhetinic acid on the 10 th day. The knowledge that Mucor Spinosus AS3. 3450 hydroxylates glycyrrhetinic acid in a site-specific position is important for future studies of the metabolism of glycyrrhetinic acid in vivo.

关 键 词：甘草次酸 刺囊毛霉 微生物转化 

分 类 号：R931.71[医药卫生—生药学] R965[医药卫生—药学]