提取何首乌不同组织总RNA的一种有效方法  

An Effective Method of Total RNA Extraction from Polygonum multiflorum Thunb. Different Tissues

作　　者：谭雪梅[1] 申彦晶[1] 赵树进[1] 

机构地区：[1]广州军区广州总医院药学部,广东广州510010

出　　处：《药物生物技术》2008年第1期24-27,共4页Pharmaceutical Biotechnology

基　　金：广东省科技厅项目(项目编号63108)

摘　　要：通过优化何首乌不同组织总RNA的提取条件,将去污剂CTAB结合酸性酚的作用,可以有效防止基因组DNA的污染;利用不同的盐浓度下,RNA与多糖在溶解度上存在差异的特点,从而溶解RNA沉淀中残留的多糖,使后者留在上清液中;碱性裂解液(pH值8.0)和高浓度巯基乙醇的作用,PVP能有效结合多酚物质。这种改进方法经济且易于控制,得到的总RNA质量较高,可直接用于随后的分子生物学操作。Optimizing the conditions of the extraction buffer, we discovered an effective method to extract total RNA from Polygonum multiflorum Thunb. different tissues. This improved CTAB method can effectively inhibited polysaccharides and polyphenols. Decontaminant （CTAB） combining with acid phenol may validly degrade genome DNA. By taking advantage of the dissimilar salt concentration, poly- saccharides were thoroughly resolved from RNA precipitation and remained in the over liquid, finally removed by centrifuge. Under the effect of alkaline buffer solution and high concentration mercaptoethanol, PVP integrated polyphenols actively. High quality RNA attained from this improved CTAB method can be directly employed to further investigate such as mRNA differential display, cDNA library construction or Northern hybridization assay.

关 键 词：何首乌 溴化十六烷三甲烷胺 RNA提取 

分 类 号：R734.2[医药卫生—肿瘤] R284.2[医药卫生—临床医学]