苏拉明对肺腺癌小鼠移植瘤的抑制作用及碱性成纤维细胞生长因子表达的影响  

Inhibitory effects of suramin on lung adencarcinoma and expression of bFGF in mice

作　　者：贺兼斌[1] 向志[1] 杨凯[1] 张平[2] 

机构地区：[1]湖南怀化市第一人民医院呼吸内科,湖南怀化418000 [2]南华大学附一医院呼吸内科,湖南衡阳421001

出　　处：《中国现代医学杂志》2008年第4期408-412,共5页China Journal of Modern Medicine

基　　金：湖南省卫生厅科研基金资助(编号C2005033)

摘　　要：目的研究苏拉明联合顺铂对肺腺癌LA795细胞T739小鼠移植瘤的抑制作用和移植瘤内bFGF表达的影响。方法复制小鼠移植瘤模型,将32只接种高转移性LA795肺腺癌细胞的T739小鼠随机分成4组:对照组、顺铂组、苏拉明组和顺铂+苏拉明组。用药干预16d,用药中观察肿瘤生长情况,于接种后24d处死各组小鼠,取出双肺和剥离皮下肿瘤,计算出肺转移发生率,计数各组小鼠肺表面转移结节数及算出肺表面结节转移抑制率。移植瘤标本行病理观察,免疫组化和图像分析系统定量检测肿瘤组织微血管密度(MVD)及碱性成纤维细胞生长因子(bFGF)表达。结果各用药组肿瘤的生长受到抑制,瘤重明显低于对照组,其抑瘤率分别为23.03%、34.40%和56.30%。苏拉明组与对照组比肺表面转移结节数、肺转移发生率下降,肺表面结节转移抑制率上升,联合组更为明显。病理观察见各用药组肿瘤细胞出现坏死、苏拉明组及联合组肿瘤间质中血管数减少。苏拉明组,顺铂+苏拉明组MVD、bFGF的表达都比对照组减少,差异有显著性(P<0.01);两者联合有协同下调MVD、bFGF表达的作用,而单用顺铂组与对照组比差异无显著性。结论苏拉明可明显抑制肺腺癌细胞在小鼠体内的生长和转移,与顺铂有协同作用,其机制可能与下调bFGF表达,抑制其微血管形成有关。[Objective] To study the effect of suramin in combination with DDP on the growth, lung metastasis and expression of bFGF of lung adenocarcinoma LA795 in vivo in mice. [Method] 32 T739 mice bearing high metastatic LA795 cells were randomly divided into four groups： control group, DDP group, suramin group, and DDP ＋ Suramin groupe. Different treatment therapy were served from day 4 after transplantation and all mice were sacrificed after 24 days. lung and subcutaneous tumors were processed for histological examination. The metastatic tumor foci on lung in mice, Lung metastatic occurring rate and metastatic foci inhibitory rate were counted. Subcutaneous tumor mierovessel density （MVD）, and the expression of basic fibroblast growth factor （bFGF） were detected by immunhistoehemieal method with image analyse system. [Results] In every treatment group, tumor growth was suppressed significantly. Intraperitoneal injection of DDP, suramin and their combination resulted in significant inhibition on the growth of LA795 cells in vivo （P 〈0.05）, the combination group showed significant increase in anti-tumor efficacy, with growth inhibition rate of 23.0%, 34.4% and 56.3%, respectively. Necrosis and decrease of vessels in the tumor and morphological changes of endothelium were observed under light microscope in suramin group and DDP ＋ Suramin group. Immunohistoehemieal staining of MVD and bFGF showed that Suramin group and DDP ＋ Suramin group were significant lower than control group （P 〈0.01）. The metastatic foei on lung surface were obviousy inhibited by suramin and suramin＋DDP compared to DDP and the control group. Contracted to DDP, the expression of subcutaneous tumor MVD, bFGF and lung metastatic occurring rate were also obviously inhibited by suramin. The combined group showed great synergetie effect in deereaseing metastatic foei on lung surface. [Conclusion] Suramin can inhibit tumor angiogenesis in lung adeneareinoma and has strong inhibit effeetory on both tumor growth and lung metastas

关 键 词：苏拉明 血管生成抑制刑 肺腺癌 肿瘤转移 

分 类 号：R73-3[医药卫生—肿瘤]