羟自由基化学发光体系测定抗坏血酸  

A novel chemiluminescence system for the determination of ascorbic acid based on hydroxyl radical reaction

作　　者：何德勇[1] 何树华[2] 章竹君[3] 

机构地区：[1]井冈山学院化学化工学院,江西吉安343009 [2]长江师范学院化学系,重庆408005 [3]西南大学化学化工学院,重庆400715

出　　处：《化学试剂》2008年第2期127-128,131,共3页Chemical Reagents

基　　金：国家自然科学基金资助项目(30470886);重庆市教委科学研究项目(KJ051305)

摘　　要：研究发现,铁（Ⅲ）与过氧化氢在酸性条件下生成羟自由基（.OH）,羟自由基（.OH）和罗丹明6G（Rh6G）混合可以产生微弱的化学发光,抗坏血酸可以增敏其微弱化学发光,结合流动注射技术,建立了一种新的抗坏血酸化学发光分析法。增敏的化学发光强度与2.0×10^-6-8.0×10^-4mg/mL范围内的抗坏血酸呈良好线性,检出限为1×10^-6mg/mL（3σ）,对5.0×10^-5mg/mL的抗坏血酸平行测定11次,其相对标准偏差为3.1%。文章最后对其反应的可能机理进行了探讨。A novel chemiluminescence （CL） reaction between the hydroxyl radical and aseorbic acid was described in this paper. The hydroxyl radical generated on line by the reaction between Fe^3 ＋ solution and H2O2 solution in the HCl medium could oxidize rhodamine 6G to produce weak chemiluminescence. It was found that ascorbic acid could enhance the chemiluminescence and the excited rhodamine 6G was the emitter of the chemiluminescence reaction. The possible mechanism of the CL system was also discussed. Ascorbic acid could be determined in a concentration range of 2.0×10^-6-8.0×10^-4 mg/mL with a detection limit of 1×10^-6mg/mL（3σ）, complete analysis could be done in 1 minute with the relative standard deviation reaching 3.1% at an ascorbic acid concentration of 5.0×10^-5mg/mL （n = 11 ）.

关 键 词：抗坏血酸 化学发光 羟自由基 罗丹明6G 

分 类 号：O657.3[理学—分析化学] O629.4[理学—化学]