马铃薯卷叶病毒复制酶基因3'端克隆及序列分析  

MOLECULAR CLONING AND SEQUENCE ANALYSIS OF 3’END OF REPLICASE GENE OF POTATO LEAFROLL VIRUS CHINESE ISOLATE.

作　　者：梁成罡[1] 哈斯阿古拉[1] 张鹤龄[1] 

机构地区：[1]内蒙古大学

出　　处：《病毒学报》1997年第3期278-282,共5页Chinese Journal of Virology

摘　　要：马铃薯卷叶病毒复制酶基因３端克隆及序列分析梁成罡哈斯阿古拉张鹤龄（内蒙古大学，呼和浩特市０１００２１）关键词马铃薯卷叶病毒，复制酶，基因克隆，ＰＣＲ马铃薯卷叶病毒（Ｐｏｔａｔｏｌｅａｆｒｏｌｖｉｒｕｓ，ＰＬＲＶ）属黄化病毒组（Ｌｕｔｅｏｖｉｒｕｓ）...The 3'-part of the replicase gene of PLRV-Ch-RNA was cloned and sequenced.Its sequence showed it consisted of 607nt including the most conservative bioactive motif'GDD'.The sequence homologies,if compared with the corresponding region of PLRV-S、-N、-C and -A,were 99.5%,99%,98.2% and 95.7% in nucleotide,and 100%,99.5%,100% and 99% in amino acid respectively. This is the first report of replicase gene of PLRV-Ch in China.

关 键 词：植物病毒 马铃薯卷叶病毒 复制酶 基因克隆 

分 类 号：S432.41[农业科学—植物病理学] S435.32[农业科学—农业昆虫与害虫防治]