猪TOLL样受体2基因在CHO-K1细胞中的表达  

Expression of porcine Toll-like receptor TLR2 gene in the CHO-K1 cells

作　　者：房永祥[1] 景志忠[2] 陈国华[2] 段凤云[2] 蒙学莲[2] 窦永喜[2] 

机构地区：[1]甘肃农业大学,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所,甘肃省动物寄生虫病重点实验室,家畜疫病病原生物学国家重点实验室,甘肃兰州730046

出　　处：《甘肃农业大学学报》2008年第1期14-17,共4页Journal of Gansu Agricultural University

基　　金：国家高技术研究发展计划“863”计划--“新型高效农业生物药物载体和新制剂创制”(2006AA10A203).

摘　　要：将含猪TLR2(猪Toll样受体2,pTLR2)基因的真核表达载体PcDNA3.1/CT-GFP-pTLR2,采用脂质体法转染到CHO-K1细胞,用G418持续筛选获得了阳性细胞克隆.阳性细胞克隆系经PCR和RT-PCR检测表明:pTLR2基因得到稳定整合并能成功地转录.荧光显微镜观察,转染细胞可见绿色荧光,表明获得了表达pTLR2的克隆细胞系.The recombinant plasmid PcDNA3. 1/CT-GFP-pTLR2 was transfected into the CHO- K1 cells by lipofectin and the positive cell clone was obtained after screening by G418 for 2-3 weeks. Analysis of the exogenous gene by PCR and RT-PCR indicated that the pTLR2 gene was integrated into the CHO-K1 cells. The expression of pTLR2-GFP fusion protein was observed by intensity fluorescence microscope successfully at different time after transfection. The results indicated that pTLR2-GFP fusion protein was expressed in the cells and these cells could be further used to investigate the functions of pTLR2.

关 键 词：猪Toll样受体2 CHO-K1细胞 转染 

分 类 号：Q786[生物学—分子生物学]