川芎油对脑缺血再灌注损伤大鼠的保护作用  

The protective effect of Chuanxiong Oil on focal cerehral reperfusion injury in rat

作　　者：凌婧[1] 邓文龙[2] 张杰[3] 杨芳炬[3] 

机构地区：[1]苏州卫生职业技术学院检验药学系,苏州2150093 [2]四川省中医药科学院,成都610041 [3]四川大学华西医学中心基础与法医学院药理教研室,成都610041

出　　处：《中药药理与临床》2008年第1期39-41,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：四川省科技厅重点课题

摘　　要：目的:观察川芎油口服给药对脑缺血再灌注损伤大鼠的保护作用。方法:线栓法建立大鼠局灶性脑缺血再灌注损伤(MCAO)模型,脑缺血2小时、再灌注后22小时检测大鼠神经功能评分和脑梗死灶体积,测定各组大鼠脑组织匀浆中一氧化氮(NO)、超氧化物歧化酶(SOD)和丙二醛(MDA)的含量。结果:川芎油能明显改善脑缺血再灌注损伤大鼠神经功能,减小脑梗死灶体积,降低脑组织匀浆中NO、MDA含量。结论:川芎油对大鼠脑缺血再灌注损伤有显著的保护作用,其作用可能与抗氧化有关。Objective： To observe the potential protective effect and possible mechanism of volatile oil of Lingusticum chuanxiong Hort（LCH） to the rats during focal cerebral ischemia-reporfusion injury. Methods： Transient middle cerebral artery （MCA） occlusion was induced as described by Longa, After 2 hours of cerebral ischemia and 24 hours of reperfusion , scores of neurological deficit were observed and the cerebral infarction size was measured by 2,3,5-triphenytetrazolium chloride （TIC） staining technique. Nitric oxide （NO） , superoxide dismutase （SOD） and malondialdehyde （MDA） levels were also observed by chemical methods. Results： Compared with MCAO model group, scores of neurological deficit and infarct volume were lower significantly in the groups treated with volatile oil, and the nitric oxide （NO） , malondialdehyde （MDA） levels were found decreased. Conclusion： The volatile oil of Ligusticum chnanxiong Holt（LCH） could protect brain damage after focal cerebral ischemia reperfusion in vivo, and the possible mechanism seemed to be that it could release the injury of fat over oxidizing.

关 键 词：川芎油 脑缺血再灌注损伤 大鼠 保护作用 抗氧化 

分 类 号：R285[医药卫生—中药学]