黄芪对早期糖尿病大鼠心肌非酶糖基化及氧化应激反应的影响  

Effect of Astragalus on nonenzymaticglycation and oxidation stress in myocardial tissue of diabetic rats

作　　者：阮耀[1] 岳兴如[1] 徐持华[1] 康爱英[1] 黄川锋[1] 

机构地区：[1]南阳医学高等专科学校药理教研室,南阳473058

出　　处：《中药药理与临床》2008年第1期47-49,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河南省科技厅科技攻关计划项目项目编号:0624240015

摘　　要：目的:研究黄芪(AS)对糖尿病大鼠心肌非酶糖基化反应和氧化应激反应的影响。方法:用链脲佐菌素腹腔注射制备糖尿病大鼠模型。黄芪注射液(10g/kg、8g/kg)ig治疗8周,分别测定各组大鼠体重、心脏质量指数、血糖、糖化血红蛋白、血清LDH、CK含量及心肌组织AGEs、MDA、NO含量及SOD、NOS活性。结果:8周实验终止时,糖尿病大鼠的心脏质量指数、血糖、糖化血红蛋白、血清LDH、CK和心肌AGEs、MDA、NO含量及NOS活性均明显高于正常组,而体重、心肌SOD活性明显低于正常组;黄芪治疗组的心脏质量指数、血糖、糖化血红蛋白、血清LDH、CK和心肌AGEs、MDA、NO含量及NOS活性均明显低于糖尿病组,但高于正常组,而体重、心肌SOD活性明显高于糖尿病组,但低于正常组。黄芪高、低剂量组间在血清LDH水平、心肌SOD活性及NO含量上有明显差异,高剂量组优于低剂量组。结论:黄芪能抑制糖尿病大鼠心肌非酶糖基化和氧化应激反应。Aim ： To investigate the effects of Astragalus on nonenzymaticglycation and oxidative stress in diabetic rats. Methods： Forty male Wistar rats were randomly divided in to four groups： normal control group （NC） , diabetic group （ DM,ip 55mg/kg STZ） , Astragalus in high doses group （ AS2 , 10g/kg per day for 8 weeks. ） and Astragalus in low doses group （ AS1, 8g/kg per day for 8 weeks. ）. At the end of 8 weeks ,the rats were killed. Body weight, heart mass/body weight, plasma glucose, glycosylated hemoglobin （HbA1c） , serum lactate dehydrogenase（LDH） and creatlne kinase（CK） were measured. Content of advancedglycationendproducts（ AGEs）, malondialdehyde （MDA） , nitric oxide （NO） and activity of superoxide dismutase （SOD） , nitric oxide synthase（NOS） in myocardial tissues were tested. Results： heart mass/body weight , plasma glucose , HbA1C , LDH and CK levels of serum , content of AGEs, MDA , NO and activity of NOS in myocardial tissues of diabetic rats were significandy higher than those of normal controls （ P 〈0.01 ）. But body weight, SOD activity of heart tissue in diabetic rats were significandy lower than those of normal controls （ P 〈 0.01 ）. After 8 weeks of treatment, heart mass/body weight , plasma glucose , HbA1 C , LDH and CK levels of serum ,content of AGEs, MDA , NO and activity of NOS in myocardial tissues of Astragalus groups were greatly lower than those of diabetic rats ,but higher than normal controls （ P 〈0.01 ,P 〈0.05）. But body weight, SOD activity of heart tissue in Astragalus groups were significantly higher than those of diabetic rats , but lower than normal controls （ P 〈 0.01, P 〈 0.05 ）. There is signiticandy difference in level of LDH, NO and activity of SOD between AS1 and AS2. Conclusion： Astragalus inhibits both nonenzymaticglycation and oxidative stress in heart of diabetic rats.

关 键 词：黄芪 糖尿病 心肌病 非酶糖基化 氧化应激反应 

分 类 号：R285[医药卫生—中药学]