豫麦18再生系统的建立及愈伤对抗生素G418抗性的研究  

Regeneration of Yumai-18 Wheat and Resistance of Callus to Antibotic G418

作　　者：押辉远[1] 谷运红[2] 焦贞[2] 

机构地区：[1]洛阳师范学院生命科学系,河南洛阳471022 [2]郑州大学离子束诱变育种及生物工程重点实验室,河南郑州450052

出　　处：《河南科技大学学报（自然科学版）》2008年第2期86-89,共4页Journal of Henan University of Science And Technology:Natural Science

基　　金：国家自然科学基金项目(10505018);河南省自然科学基金项目(511030400)

摘　　要：以小麦品种豫麦18为受体进行外源基因转移,以豫麦18的成熟胚为外植体进行愈伤诱导,通过分化培养基的分化获得小麦幼苗,建立了豫麦18再生系统。同时,通过实验认为,培养基中25mg/L的G418可以作为筛选转基因抗性愈伤的浓度。In order to introduce foreign DNA fragment into wheat yumai-18 planted popularly in Henan province,the callus were induced from mature embryo of wheat yumai-18,and then,the regenerated plant was obtained by differention culture,so the regeneration system was obtained.On the other hand,the untransgenic wheat cullus were killed by the antibotic G418 at the concerntation of the 25 mg/L.

关 键 词：小麦基因转化 豫麦18 愈伤诱导 抗性愈伤 

分 类 号：S512.1[农业科学—作物学]