IL-17参与EAU发病及其作用探讨  

Involvement of IL-17 in EAU pathogenesis

作　　者：钱丽丽[1] 张凤蕴[1] 郭涛[1] 黄琪[1] 赵瑞波[1] 王丽群[1] 任欢[1] 

机构地区：[1]哈尔滨医科大学免疫学教研室,150081

出　　处：《国际免疫学杂志》2008年第2期86-88,120,F0003,共5页International Journal of Immunology

摘　　要：目的诱导建立大鼠EAU模型，探讨Th17是否参与EAU发病及其可能的作用机制。方法用视网膜S抗原与Freund完全佐剂免疫大鼠诱导EAU模型。观察EAU发作时间、EAU临床评分及组织病理学改变，并采用免疫组织化学SP法，检测EAU组和对照组动物眼后部葡萄膜视网膜IL-17的表达。结果EAU组动物的眼睛在免疫后不同时间发生不同程度的炎症（发病率为100％），临床评分为（3．14±0．61），具有阳性体征者均有葡萄膜视网膜组织病理学改变，模型建立成功。经免疫组织化学染色发现，EAU组IL-17表达阳性率为86％。结论Th17在EAU的发病机制中可能发挥一定作用。Objective To induce experimental autoimmune uveoretinitis （EAU） for discuss if Th17 involve in EAU pathogenesis and the possible function mechanism of it. Methods EAU was induced by immunization with retinal S-antigen and complete Freund＇s adjuvant. Immunohistochemistry was employed to detect the expression of IL-17 in both EAU group and control group. Results EAU was induced after immunization with S-Ag（ The mobility was 100% ） ,clinical scoring is 3.14 ±0.61, Rat EAU models were constructed in 14 out of 14 immunized animals, with which clinical scoring were applied. Expression of IL-17 was detected in EAU group by Immunohistochemistry. Conclusion Th17 may play certain role in the pathogenesis of EAU.

关 键 词：实验性自身免疫性葡萄膜视网膜炎(EAU) 发病机制 IL-17 

分 类 号：R774.1[医药卫生—眼科]