As4S4诱导宫颈癌Hela细胞凋亡及对端粒酶活性的影响  

Study on As4S4 induced apoptosis and its effect on telomerase activity in human cervical cancer cells Hela

作　　者：尹伶[1] 濮德敏[2] 韦立蓓[1] 瞿秋红[1] 

机构地区：[1]武汉科技大学临床学院妇产科,湖北武汉430064 [2]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030

出　　处：《中国现代医学杂志》2008年第3期298-301,共4页China Journal of Modern Medicine

摘　　要：目的研究雄黄主要成分硫化砷(As4S4)抑制宫颈癌Hela细胞株增殖和诱导凋亡时对细胞内端粒酶活性的影响。方法用不同浓度的As4S4作用于Hela细胞株不同时间段后光镜观察细胞形态变化;四甲基偶氮唑蓝(MTT)法测定细胞增殖抑制率;流式细胞仪(FCM)检测细胞凋亡率;DNA梯状电泳(DNAladder)检测凋亡的发生;PCR-ELISA法测定细胞内端粒酶活性变化。结果As4S4可抑制Hela细胞增殖,作用呈明显的时效和量效关系,24hIC50为30mg/L;As4S4诱导Hela细胞的凋亡率明显增加并呈浓度依赖性;DNAlad-der呈梯状条带;细胞内端粒酶活性明显受抑制。结论As4S4具有抑制Hela细胞增殖和促进凋亡作用,其机制可能与抑制细胞内端粒酶活性有关。[Objective] To investigate the Hela ceils growth inhibition and apoptosis induced by As4S4 and its relationship with telomerase activity. [Methods] Hela cells were treated with As4S4 in various concentrations with varions durations. Cell growth was measured by MTI＇. Apoptosis was detected by double staining flow cytometry （FCM） and DNA ladder. Telomerase activity in ceils was measured by PCR-ELISA. [Results] After being treated with different concentrations of As4S4, the cell growth of Hela was suppressed significantly in a dose-and time-dependent manner,24 h IC50 was 30mg/L. The apoptotic rate was increased obviously in a dose-dependent manner by As4S4. DNA fragmentation gel analysis showed DNA ladder. Telomerase activity in ceils was inhibited obviously. [Conclusion] As4S4 can inhibit the proliferation and increase apoptosis in human Hela ceils. Its mechanism may depend on the inhibition of the telomerase activity.

关 键 词：AS4S4 端粒酶 HELA细胞 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]