2-氯酚驯化对厌氧污泥微生物种群结构的影响  

Effects of 2-chlorophenol-acclimation on Microbial Community Structure in Anaerobic Granular Sludge

作　　者：黄爱群[1] 戴亚蕾[2] 陈玲[1] 陈皓[1] 张文[1] 

机构地区：[1]同济大学环境学院污染控制与资源化研究国家重点实验室,上海200092 [2]同济大学医学院免疫实验室,上海200092

出　　处：《环境科学》2008年第3期781-787,共7页Environmental Science

基　　金：国家高技术研究发展计划(863)项目(20025AA601270)

摘　　要：用古细菌特异性引物ARC21F/ARC958R和酸杆菌特异性引物31F/907R,对2-氯酚(2-chlorophenol,2-CP)驯化前后厌氧颗粒污泥样品总DNA进行了16SrDNA的聚合酶链式反应(polymerase chain reaction,PCR)、克隆、测序及序列分析,研究了2-CP驯化前后厌氧颗粒污泥中微生物种群结构的变化.结果表明,2-CP驯化前后厌氧颗粒污泥中存在共有的古细菌菌种:Methanothrix soehngenii、Methanosaeta concilii及uncultured euryarchaeote等.2-CP驯化后的厌氧污泥中出现了特有的微生物Methanobacterium aarhusense、Methanobacterium curvum及Methanobacterium beijingense等,而接种污泥中特有的一些古细菌发生了消亡.对该厌氧体系酸杆菌的分析表明,2-CP驯化前后的厌氧颗粒污泥中存在共有的微生物菌种:uncultured bacterium、uncultured Acidobacterium和unknown Actinomycete(MC 9).经2-CP驯化后,接种污泥中特有的微生物Desulfotomaculum sp.176、uncultured Deltaproteobacterium n8d及uncultured hydrocarbon seep bacterium等发生消亡,并出现了特有的微生物unculturedHolophaga/Acidobacterium、uncultured Acidobacteria bacterium及unidentified Acidobacterium.The microbial community structure in 2-chlorophenol-acclimated anaerobic granular sludge and inoculating sludge were analyzed by 16S rDNA-based approach. Total DNA was extracted directly from the inoculating sludge and 2-CP-acclimated anaerobic sludge, and then amplified by polymerase chain reaction （PCR） technique with the specific primer pair ARC21F/ARC958R for Archaea and 31F/907R for Acidobacteria respectively. The positive PCR products were cloned and sequenced. The sequences analysis shows that there exist common Archaea in both sludge, including Methanothrix soehngenii, Methanosaeta concilii and uncultured euryarchaeote etc. Some special Archaea appear in the 2-CP-acclimated sludge, such as Methanobacterium aarhusense, Methanobacterium curvum and Methanobacterium beijingense etc. Others originally existed in the inoculating sludge disappear after acclimation. Common Acidobacteria are found in both sludge, including uncultured bacterium, uncultured Acidobacterium and unknown Actinomycete （MC 9）. Some special microbes originally existed in the inoculating sludge, such as Desulfotomaculum sp. 176, uncultured Deltaproteobacterium n8d and uncultured hydrocarbon seep bacterium etc. disappear after acclimation, and uncultured Holophaga/Acidobacterium, uncultured Acidobacteria bacterium and unidentified Acidobacterium are found after 2-CP-acclimation.

关 键 词：2-氯酚 厌氧颗粒污泥 驯化 古细菌 酸杆菌 

分 类 号：X172[环境科学与工程—环境科学]