澳洲青苹离体叶片不定芽再生体系的建立  

Adventitious Shoot Regeneration from Leaves of Granny Smith Apple in vitro

作　　者：李玉玲[1] 王三红[1] 刘莉莉[1] 章镇[1] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095

出　　处：《江苏农业学报》2008年第1期48-52,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(30370987);江苏省应用项目(BJ200018)

摘　　要：以澳洲青苹试管苗的叶片为外植体诱导不定芽再生,研究不同的BA浓度、TDZ浓度、暗培养时间、叶片放置方式和AgNO3对叶片不定芽再生的影响。结果表明:最适宜的叶片不定芽再生的培养基为MS+TDZ 2.0mg/L+NAA 0.3 mg/L+琼脂6.0 g/L+蔗糖30.0 g/L和MS+BA 4.0 mg/L+NAA 0.2 mg/L+琼脂6.0 g/L+蔗糖30.0 g/L,最适的暗培养时间是20 d,在上述2种培养基中澳洲青苹离体叶片不定芽的再生频率分别达到100.0%和91.7%。Shoot regeneration was conducted with leaves of test-tube plantlets of granny smith apple to study the effects of different concentrations of AB, TDZ, dark incubation time, leaf burl and AgNO3 on regeneration in vitro. The result showed that the optimum medium was MS ＋ TDZ 2. 0 mg/L ＋ NAA 0. 3 mg/L ＋ sucrose 30. 0 g/L ＋ agar 6. 0 g/L or MS ＋ BA 4. 0 mg/L ＋ NAA 0. 2 mg/L ＋ sucrose 30. 0 g/L ＋ agar 6. 0 g/L. The optimum dark incubation time was 20 d.

关 键 词：苹果 叶片 TDZ BA 不定芽再生 

分 类 号：S661.103[农业科学—果树学]